Sarbia M, Bösing N, Hildebrandt B, Koldovsky P, Gerharz C D, Gabbert H E
Institute of Pathology, Heinrich-Heine-University, Düsseldorf, Germany.
Anticancer Res. 1997 May-Jun;17(3C):2185-92.
This study describes characteristics of two newly established cell lines (OSC-1 and OSC-2), derived from two oesophageal squamous cell carcinomas. Morphologically, OSC-1 cells and OSC-2 cells grew in epithelial cobblestone patterns with cells piling up to 4 cells. Ultrastructurally, both cell lines showed formation of desmosomes; however, tonofilaments were only formed by OSC-2 cells. Immunohistochemical investigations revealed coexpression of vimentin and cytokeratin in OSC-1 cells and OSC-2 cells. A cytokeratin subtype typical for mature squamous epithelia (cytokeratin 13) was expressed only in OSC-2 cells. OSC-1 cells showed tumour formation in nude mice, whereas OSC-2 cells did not. Cytogenetic analysis revealed that OSC-1 cells had a hyperdiploid karyotype and OSC-2 cells had a near-triploid karyotype. In both cell lines, the formation of multicellular spheroids could be induced. In conclusion, in comparison with OSC-2 cells the OSC-1 cells were characterized by a poorer degree of differentiation and by a more aggressive growth behaviour in vivo.
本研究描述了源自两例食管鳞状细胞癌的两个新建立的细胞系（OSC-1和OSC-2）的特征。形态学上，OSC-1细胞和OSC-2细胞呈上皮鹅卵石样生长，细胞堆积达4层。超微结构上，两个细胞系均显示有桥粒形成；然而，张力丝仅在OSC-2细胞中形成。免疫组织化学研究显示，波形蛋白和细胞角蛋白在OSC-1细胞和OSC-2细胞中共表达。成熟鳞状上皮典型的细胞角蛋白亚型（细胞角蛋白13）仅在OSC-2细胞中表达。OSC-1细胞在裸鼠中形成肿瘤，而OSC-2细胞则不形成。细胞遗传学分析显示，OSC-1细胞具有超二倍体核型，OSC-2细胞具有近三倍体核型。在两个细胞系中均可诱导多细胞球体的形成。总之，与OSC-2细胞相比，OSC-1细胞的特征是分化程度较差，在体内生长行为更具侵袭性。
