Cytogenetics Department, Plant Breeding Institute, Cambridge, England.
Theor Appl Genet. 1975 Jan;45(7):315-21. doi: 10.1007/BF00276686.
Mitochondria isolated from etiolated shoots of a range of maize genotypes with the "Texas" cytoplasm conferring cytoplasmically-inherited male sterility, are sensitive to a pathotoxin isolated from Helmintho-sporium maydis, race T. The pathotoxin inhibits oxidation of α ketoglutarate and malate and stimulates NADH oxidation. The time taken for the pathotoxin to induce these changes is a measure of the sensitivity of the mitochondria to the pathotoxin. A range of nine different pairs of genotypes, each pair differing principally in the presence of nuclear male fertility restorer alleles has been compared in their sensitivity to pathotoxin. In every case the line carrying the restorer alleles is more resistant to the pathotoxin. The restored genotypes can be quantitatively arranged into groups which correspond to the four different sources of the restorer genes in these lines. It is suggested that the restorer genes cause changes in mitochondria, which modify the functional aberration introduced by the cytoplasmically-inherited mutation causing sterility.
从具有“德克萨斯”细胞质的一系列玉米基因型的黄化芽中分离的线粒体对来自 Helmintho-sporium maydis，race T 的病理毒素敏感。该病理毒素抑制α酮戊二酸和苹果酸的氧化，并刺激 NADH 氧化。病理毒素诱导这些变化所需的时间是衡量线粒体对病理毒素敏感性的指标。已经比较了一系列 9 对不同的基因型，每对基因型主要在核雄性育性恢复等位基因的存在上存在差异，以比较它们对病理毒素的敏感性。在每种情况下，携带恢复等位基因的系对病理毒素的抗性更强。恢复的基因型可以定量排列成与这些系中恢复基因的四个不同来源相对应的组。有人认为，恢复基因引起线粒体的变化，从而修饰由导致不育的细胞质遗传突变引入的功能异常。
