一株新分离的嗜碱芽孢杆菌 41M-1 产碱性木聚糖酶的研究。
Production of alkaline xylanase by a newly isolated alkaliphilic Bacillus sp. strain 41M-1.
机构信息
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo University of Technology, 4259 Nagatsuta, Midori-ku, 227, Yokohama, Japan.
出版信息
World J Microbiol Biotechnol. 1993 Mar;9(2):221-4. doi: 10.1007/BF00327842.
Alkaliphilic Bacillus sp. strain 41M-1, isolated from soil, produced xylan-degrading enzymes extracellularly. Optimum pH for the crude xylanase preparation was about pH 9, confirming the production of novel alkaline xylanase(s) by the isolate. Xylanases were induced by xylan, but were not produced in the presence of xylose, arabinose or glucose. Xylanase productivity was influenced by culture pH, and production at pH 10.5 was higher than that at pH 8.0. Zymogram analysis of the culture supernatant showed the alkaline xylanase with a molecular mass of 36 kDa.
从土壤中分离到的嗜碱性芽孢杆菌 41M-1 株能够分泌胞外木聚糖酶。粗酶制剂的最适 pH 值约为 9，表明该菌产生了新型碱性木聚糖酶。木聚糖诱导木聚糖酶的产生，但木糖、阿拉伯糖或葡萄糖的存在不会诱导木聚糖酶的产生。木聚糖酶的产生受培养 pH 值的影响，在 pH 值 10.5 下的产量高于 pH 值 8.0 下的产量。培养上清液的同工酶分析表明，该碱性木聚糖酶的分子量为 36 kDa。
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