De Kruyff R H, Ju S T, Laning J, Dorf M E
Eur J Immunol. 1987 Aug;17(8):1115-20. doi: 10.1002/eji.1830170807.
The synthetic random copolymer of L-glutamic acid and L-lysine (GL) is weakly or nonimmunogenic in all inbred strains of mice. Theories proposed to account for nonresponsiveness to GL include a deficient T cell repertoire, failure of antigen-presenting cells to present the antigen and/or the presence of suppressor cells. In this study we examine mechanisms for nonresponsiveness to GL. We demonstrate the existence of GL-reactive T cells which can be isolated with a relatively high frequency. These clones, which were derived following immunization of H-2d mice with poly(LGluLLysLTyr), also respond to several GL-containing polypeptides including the terpolymers of GL with phenylalanine, alanine (GLA) or leucine. Although recognition of GLA by heterogeneous T cell populations usually occurs in association with I-A determinants, these clones recognize GLA, as well as the other GL-containing polymers, in association with I-E determinants. Analysis of the antigen and alloreactivity patterns of these clones indicated that they expressed distinct antigen receptors. These studies imply that the T cell repertoire of "nonresponder" H-2d mice includes multiple GL-reactive T cell clones and that the antigen-presenting cells of these mice are effective in processing and presenting GL.
L-谷氨酸和L-赖氨酸的合成随机共聚物（GL）在所有近交系小鼠中具有弱免疫原性或无免疫原性。提出的解释对GL无反应性的理论包括T细胞库缺陷、抗原呈递细胞无法呈递抗原和/或抑制细胞的存在。在本研究中，我们研究了对GL无反应性的机制。我们证明了存在可以相对高频率分离的GL反应性T细胞。这些克隆是在用聚（LGluLLysLTyr）免疫H-2d小鼠后获得的，它们也对几种含GL的多肽有反应，包括GL与苯丙氨酸、丙氨酸（GLA）或亮氨酸的三元共聚物。虽然异质性T细胞群体对GLA的识别通常与I-A决定簇相关，但这些克隆识别GLA以及其他含GL的聚合物时与I-E决定簇相关。对这些克隆的抗原和同种异体反应性模式的分析表明它们表达了不同的抗原受体。这些研究表明，“无反应性”H-2d小鼠的T细胞库包括多个GL反应性T细胞克隆，并且这些小鼠的抗原呈递细胞在处理和呈递GL方面是有效的。
