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World J Microbiol Biotechnol. 1994 Mar;10(2):159-64. doi: 10.1007/BF00360878.
Freshwaters of varying natural nutrient enrichment were used as growth media for the culture of an autochthonous, heterotrophic, freshwater bacterium, Aeromonas hydrophila. The growth rate of the bacterium in eutrophic waters was increased to the greatest extent by adding carbon, as glucose; generation times decreased by up to 65%. Additions of carbon and phosphorus increased the maximal cell densities by over 25-fold. In oligotrophic waters, bacterial growth was most strongly promoted by the simultaneous additions of carbon (as glucose) and phosphorus (as KH2PO4). In these waters, stationary phase densities were increased as much as 100-fold, with a corresponding 70% increase in growth rate. These data provide at least a partial explanation for the previously observed correlation between A. hydrophila densities and the trophic states of freshwaters.
采用不同自然富营养化程度的淡水作为培养基,培养一种土著异养型淡水细菌,嗜水气单胞菌。在富营养化水体中,通过添加葡萄糖等碳源,细菌的生长速度最大程度地提高,世代时间最多减少了 65%。添加碳和磷可使最大细胞密度增加 25 倍以上。在贫营养化水体中,同时添加碳(如葡萄糖)和磷(如 KH2PO4)最能促进细菌生长。在这些水体中,静止期密度增加了 100 倍,生长速度相应增加了 70%。这些数据至少部分解释了先前观察到的嗜水气单胞菌密度与淡水营养状态之间的相关性。