van der Kooij D, Hijnen W A
Netherlands Waterworks' Testing and Research Institute, KIWA Ltd., Nieuwegein.
Appl Environ Microbiol. 1988 Nov;54(11):2842-51. doi: 10.1128/aem.54.11.2842-2851.1988.
The nutritional versatility and growth kinetics of Aeromonas hydrophila were studied to determine the nature and the growth-promoting properties of organic compounds which may serve as substrates for the growth of this organism in drinking water during treatment and distribution. As an initial screening, a total of 69 different organic compounds were tested at a concentration of 2.5 g/liter as growth substrates for 10 A. hydrophila strains. Of these strains, strain M800 attained the highest maximum colony counts in various types of drinking water and river water and was therefore used in further measurements of growth at low substrate concentrations. A mixture of 21 amino acids and a mixture of 10 long-chain fatty acids, when added to drinking water, promoted growth of strain M800 at individual compound concentrations as low as 0.1 microgram of C per liter. Mixtures of 18 carbohydrates and 18 carboxylic acids clearly enhanced growth of the organism at individual compound concentrations above 1 microgram of C per liter. Growth measurements with 63 individual substrates at a concentration of 10 micrograms of C per liter gave growth rates of greater than or equal to 0.1/h with two amino acids, nine carbohydrates, and six long-chain fatty acids. Ks values were determined for arginine (less than or equal to 0.3 micrograms of C per liter), glucose (15.9 micrograms of C per liter), acetate (11.1 micrograms of C per liter), and oleate (2.1 micrograms of C per liter). The data obtained indicate that biomass components, such as amino acids and long-chain fatty acids, can promote multiplication of aeromonads in drinking water distribution systems at concentrations as low as a few micrograms per liter.
研究嗜水气单胞菌的营养多样性和生长动力学，以确定在饮用水处理和分配过程中可作为该生物体生长底物的有机化合物的性质和促生长特性。作为初步筛选，以2.5 g/升的浓度测试了总共69种不同的有机化合物作为10株嗜水气单胞菌菌株的生长底物。在这些菌株中，M800菌株在各种类型的饮用水和河水中获得了最高的最大菌落数，因此用于进一步测量低底物浓度下的生长情况。当将21种氨基酸的混合物和10种长链脂肪酸的混合物添加到饮用水中时，即使单个化合物浓度低至每升0.1微克碳，也能促进M800菌株的生长。18种碳水化合物和18种羧酸的混合物在单个化合物浓度高于每升1微克碳时明显增强了该生物体的生长。以每升10微克碳的浓度对63种单个底物进行生长测量，结果显示两种氨基酸、九种碳水化合物和六种长链脂肪酸的生长速率大于或等于0.1/h。测定了精氨酸（小于或等于每升0.3微克碳）、葡萄糖（每升15.9微克碳）、乙酸盐（每升11.1微克碳）和油酸酯（每升2.1微克碳）的Ks值。所获得的数据表明，生物质成分，如氨基酸和长链脂肪酸，在浓度低至每升几微克时就能促进饮用水分配系统中气单胞菌的繁殖。
