耐抗生素性和耐药性-结节性扩散泵-和外膜孔蛋白编码基因在加拿大医院分离的不动杆菌属物种中的表达。

Antibiotic resistance and expression of resistance-nodulation-division pump- and outer membrane porin-encoding genes in Acinetobacter species isolated from Canadian hospitals.

机构信息

Antimicrobial Resistance Research Group, Medical Laboratory Science and Applied Bioscience Programs, Faculty of Health Sciences, University of Ontario Institute of Technology, Oshawa, Ontario;

Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba.

出版信息

Can J Infect Dis Med Microbiol. 2013 Spring;24(1):17-21. doi: 10.1155/2013/696043.

DOI:10.1155/2013/696043

PMID:24421787

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3630023/

Abstract

BACKGROUND

Bacterial pathogens belonging to the genus Acinetobacter cause serious infections in immunocompromised individuals that are very difficult to treat due to their extremely high resistance to many antibiotics.

OBJECTIVE

To investigate the role of resistance-nodulation-division (RND) pumps and porins in the antibiotic resistance of Acinetobacter species collected from Canadian hospitals.

METHODS

Clinical isolates of Acinetobacter species collected from Canadian hospitals were analyzed for the expression of genes encoding RND pumps (adeB, adeG, adeJ, AciBau_2746 and AciBau_2436) and outer membrane porins (carO, 33 kDa porin and oprD) using quantitative reverse transcription (qRT) polymerase chain reaction. Species identification of the isolates was performed using a multiplex polymerase chain reaction method for gyrB.

RESULTS

The expression of RND pump-encoding genes was widespread in the clinical isolates of Acinetobacter species, with each of the isolates expressing at least one RND pump. adeG was found to be overexpressed in all of the isolates, while adeB was found to be overexpressed in only two isolates. Among the porin-encoding genes, the expression of carO was considerably downregulated among the majority of isolates.

CONCLUSION

The present study was the first to analyze the expression of RND pump- and porin-encoding genes in the clinical isolates of Acinetobacter species from Canadian hospitals. The overexpression of genes encoding RND pumps and the downregulation of genes encoding porins was common in clinical isolates of Acinetobacter species from Canadian hospitals, with the AdeFGH pump being the most commonly expressed RND pump.

摘要

背景

属于不动杆菌属的细菌病原体在免疫功能低下的个体中引起严重感染，由于它们对许多抗生素具有极高的耐药性，因此非常难以治疗。

目的

研究来自加拿大医院的不动杆菌属细菌的耐药性中，耐药-结节-分裂（RND）泵和孔蛋白的作用。

方法

使用定量逆转录（qRT）聚合酶链反应分析来自加拿大医院的不动杆菌属临床分离株中编码 RND 泵（adeB、adeG、adeJ、AciBau_2746 和 AciBau_2436）和外膜孔蛋白（carO、33 kDa 孔蛋白和 oprD）的基因的表达。使用gyrB 的多重聚合酶链反应方法对分离株进行种属鉴定。

结果

RND 泵编码基因的表达在不动杆菌属临床分离株中广泛存在，每个分离株至少表达一种 RND 泵。adeG 在所有分离株中均过度表达，而 adeB 仅在两个分离株中过度表达。在孔蛋白编码基因中，carO 的表达在大多数分离株中显著下调。

结论

本研究首次分析了来自加拿大医院的不动杆菌属临床分离株中 RND 泵和孔蛋白编码基因的表达。RND 泵编码基因的过度表达和孔蛋白编码基因的下调在加拿大医院的不动杆菌属临床分离株中很常见，其中 AdeFGH 泵是最常表达的 RND 泵。

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J Antimicrob Chemother. 2012 Mar;67(3):569-72. doi: 10.1093/jac/dkr519. Epub 2011 Dec 6.

Acinetobacter baumannii and Acinetobacter genospecies 13TU and 3 bacteraemia: comparison of clinical features, prognostic factors and outcomes.

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Activity of the efflux pump inhibitor phenylalanine-arginine β-naphthylamide against the AdeFGH pump of Acinetobacter baumannii.

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耐抗生素性和耐药性-结节性扩散泵-和外膜孔蛋白编码基因在加拿大医院分离的不动杆菌属物种中的表达。

Antibiotic resistance and expression of resistance-nodulation-division pump- and outer membrane porin-encoding genes in Acinetobacter species isolated from Canadian hospitals.

机构信息

Antimicrobial Resistance Research Group, Medical Laboratory Science and Applied Bioscience Programs, Faculty of Health Sciences, University of Ontario Institute of Technology, Oshawa, Ontario;

Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba.

出版信息

Can J Infect Dis Med Microbiol. 2013 Spring;24(1):17-21. doi: 10.1155/2013/696043.

DOI:10.1155/2013/696043

PMID:24421787

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3630023/

Abstract

BACKGROUND

OBJECTIVE

To investigate the role of resistance-nodulation-division (RND) pumps and porins in the antibiotic resistance of Acinetobacter species collected from Canadian hospitals.

METHODS

RESULTS

CONCLUSION

摘要

背景

属于不动杆菌属的细菌病原体在免疫功能低下的个体中引起严重感染，由于它们对许多抗生素具有极高的耐药性，因此非常难以治疗。

目的

研究来自加拿大医院的不动杆菌属细菌的耐药性中，耐药-结节-分裂（RND）泵和孔蛋白的作用。

耐抗生素性和耐药性-结节性扩散泵-和外膜孔蛋白编码基因在加拿大医院分离的不动杆菌属物种中的表达。

Antibiotic resistance and expression of resistance-nodulation-division pump- and outer membrane porin-encoding genes in Acinetobacter species isolated from Canadian hospitals.

机构信息

出版信息

BACKGROUND

OBJECTIVE

METHODS

RESULTS

CONCLUSION

背景

目的

方法

结果

结论

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耐抗生素性和耐药性-结节性扩散泵-和外膜孔蛋白编码基因在加拿大医院分离的不动杆菌属物种中的表达。

Antibiotic resistance and expression of resistance-nodulation-division pump- and outer membrane porin-encoding genes in Acinetobacter species isolated from Canadian hospitals.

机构信息

出版信息

BACKGROUND

OBJECTIVE

METHODS

RESULTS

CONCLUSION

背景

目的

方法

结果

结论