从免疫印迹中消除人为的65 kDa角蛋白条带的检测。

Elimination of the detection of an artefactual 65 kDa keratin band from immunoblots.

作者信息

Shapiro S Z

出版信息

J Immunol Methods. 1987 Aug 24;102(1):143-6. doi: 10.1016/s0022-1759(87)80019-4.

Abstract

Contamination of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) samples with skin keratin has produced a recurrent problem of artefactual bands when highly sensitive detection methods, such as silver staining, have been used. Such an artefact also can be a problem in immunoblots because some sera appear to contain antibodies that react with a human skin keratin. A simple method for the removal of these antibodies with keratin immobilized on an affinity chromatography substrate is described.

摘要

在使用高灵敏度检测方法（如银染）时，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）样品被皮肤角蛋白污染会产生假象条带这一反复出现的问题。这种假象在免疫印迹中也可能是个问题，因为一些血清似乎含有与人皮肤角蛋白反应的抗体。本文描述了一种用固定在亲和层析基质上的角蛋白去除这些抗体的简单方法。