向多嵌段共聚物中添加聚丙二醇以优化小干扰RNA的递送。
Addition of poly (propylene glycol) to multiblock copolymer to optimize siRNA delivery.
作者信息
Dai Zhi, Arévalo Maria T, Li Junwei, Zeng Mingtao
机构信息
Center of Excellence for Infectious Diseases; Department of Biomedical Sciences; Paul L Foster School of Medicine; Texas Tech University Health Sciences Center; El Paso, TX USA.
出版信息
Bioengineered. 2014 Jan-Feb;5(1):30-7. doi: 10.4161/bioe.27339. Epub 2013 Dec 18.
Previous studies have examined different strategies for siRNA delivery with varying degrees of success. These include use of viral vectors, cationic liposomes, and polymers. Several copolymers were designed and synthesized based on blocks of poly(ethylene glycol) PEG, poly(propylene glycol) PPG, and poly(l-lysine). These were designated as P1, P2, and P3. We studied the copolymer self-assembly, siRNA binding, particle size, surface potential, architecture of the complexes, and siRNA delivery. Silencing of GFP using copolymer P3 to deliver GFP-specific siRNA to Neuro-2a cells expressing GFP was almost as effective as using Lipofectamine 2000, with minimal cytotoxicity. Thus, we have provided a new copolymer platform for siRNA delivery that we can continue to modify for improved delivery of siRNA in vitro and eventually in vivo.
先前的研究已经考察了不同的小干扰RNA（siRNA）递送策略，取得了不同程度的成功。这些策略包括使用病毒载体、阳离子脂质体和聚合物。基于聚乙二醇（PEG）、聚丙二醇（PPG）和聚L-赖氨酸的嵌段设计并合成了几种共聚物。这些共聚物被命名为P1、P2和P3。我们研究了共聚物的自组装、siRNA结合、颗粒大小、表面电位、复合物的结构以及siRNA递送。使用共聚物P3将绿色荧光蛋白（GFP）特异性siRNA递送至表达GFP的Neuro-2a细胞，对GFP的沉默效果几乎与使用Lipofectamine 2000一样有效，且细胞毒性最小。因此，我们提供了一个新的用于siRNA递送的共聚物平台，我们可以继续对其进行修饰，以改善siRNA在体外乃至最终在体内的递送效果。
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