Sgamma Tiziana, Jackson Alison, Muleo Rosario, Thomas Brian, Massiah Andrea
School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, West Midlands CV4 7AL, UK.
Department of Agriculture, Forests, Nature and Energy, University of Tuscia, Via San Camillo de Lellis snc, Viterbo 01100, Italy.
Sci Rep. 2014 Jan 15;4:3704. doi: 10.1038/srep03704.
Many plants are incapable of flowering in inductive daylengths during the early juvenile vegetative phase (JVP). Arabidopsis mutants with reduced expression of TEMPRANILLO (TEM), a repressor of flowering locus T (FT) had a shorter JVP than wild-type plants. Reciprocal changes in mRNA expression of TEM and FT were observed in both Arabidopsis and antirrhinum, which correlated with the length of the JVP. FT expression was induced just prior to the end of the JVP and levels of TEM1 mRNA declined rapidly at the time when FT mRNA levels were shown to increase. TEM orthologs were isolated from antirrhinum (AmTEM) and olive (OeTEM) and were expressed most highly during their juvenile phase. AmTEM functionally complemented AtTEM1 in the tem1 mutant and over-expression of AmTEM prolonged the JVP through repression of FT and CONSTANS (CO). We propose that TEM may have a general role in regulating JVP in herbaceous and woody species.
许多植物在幼年早期营养生长阶段（JVP）无法在诱导性光周期下开花。开花位点T（FT）的抑制因子TEMPRANILLO（TEM）表达降低的拟南芥突变体的JVP比野生型植物短。在拟南芥和金鱼草中均观察到TEM和FT的mRNA表达的相互变化，这与JVP的长度相关。FT表达在JVP结束前被诱导，并且在FT mRNA水平显示增加时，TEM1 mRNA水平迅速下降。从金鱼草（AmTEM）和橄榄（OeTEM）中分离出TEM直系同源物，并且在它们的幼年阶段表达最高。AmTEM在tem1突变体中功能性互补AtTEM1，并且AmTEM的过表达通过抑制FT和CONSTANS（CO）延长了JVP。我们提出，TEM可能在调节草本和木本植物的JVP中具有普遍作用。
