光抑制的恢复：光的作用和对 32kD 叶绿体蛋白合成的抑制。

Recovery from photoinhibition: effect of light and inhibition of protein synthesis of 32-kD chloroplast protein.

机构信息

Department of Biology, The Phytotron, University of Oslo, Blindern, P.O. Box 1066, 0316, Oslo 3, Norway.

出版信息

Photosynth Res. 1989 Sep;21(3):171-9. doi: 10.1007/BF00037181.

Abstract

Recovery from photoinhibition of photosynthesis in intact Lemna gibba was studied in presence of the protein synthesis inhibitors chloramphenicol and cycloheximide. Exposure to an irradiance of 1000 μmol m(-2)s(-1) in N2 for 90 min induced 80% photoinhibition. The plants recovered photosynthesis when transfered to normal irradiances (210 μmol m(-2)s(-1)) and air. Chloramphenicol added to the medium was taken up by the plant and reduced photosynthesis slightly. Recovery from photoinhibition was more inhibited than photosynthesis. Cycloheximide was also taken up by the plants and reduced synthesis of light harvesting chlorophyll protein: however, neither photosynthesis nor recovery were much affected. Synthesis of 32-kD chloroplast protein during recovery was inhibited by chloramphenicol, but not by cycloheximide. Synthesis of 32-kD protein was enhanced by 20-210 μmol m(-2)s(-1) light. The results support the hypothesis that synthesis of 32-kD protein is important for recovery of photosynthesis after photoinhibition.

摘要

在含有蛋白质合成抑制剂氯霉素和环己亚胺的条件下，研究了完整浮萍光合作用光抑制恢复。在 N2 中 1000 μmol m(-2)s(-1)的辐照度下暴露 90 分钟会导致 80%的光抑制。当将植物转移到正常辐照度（210 μmol m(-2)s(-1)）和空气中时，光合作用会恢复。添加到培养基中的氯霉素被植物吸收，并使光合作用略有降低。光抑制的恢复比光合作用的恢复受到更大的抑制。环己亚胺也被植物吸收并减少了光捕获叶绿素蛋白的合成：然而，光合作用和恢复都没有受到太大影响。在恢复过程中，32-kD 叶绿体蛋白的合成被氯霉素抑制，但不受环己亚胺的影响。20-210 μmol m(-2)s(-1)的光会增强 32-kD 蛋白的合成。这些结果支持了这样的假设，即 32-kD 蛋白的合成对于光抑制后光合作用的恢复是重要的。