Institute of General Genetics, University of Oslo, Blindern, Box 1031, Oslo 3, Norway.
Planta. 1976 Jan;130(3):327-32. doi: 10.1007/BF00387841.
Soluble protein, about 15% of the total cellular protein, was extracted from different stages in the haplodiplontic life cycle of Ulva mutabilis. The electrophoretic band pattern of the protein extracts from the haploid gametophyte and the diploid sporophyte were found to be the same, except for one slow moving band present in the gametophyte, but lacking in the diploid sporophyte. This band was also missing in the extract from the haploid parthenosporophyte, but is seen in the extract from the zoospores. It was found that the synthesis of the protein in this band occurred during most of the preparation period preceding meiosis. The band is not seen in extracts from gametes, and it is inferred that this protein is broken down during the period preceding the mitotic gametangial division in the gametophyte. So far the protein making up this band behaves as should be required for a factor determining the shift in generation during the life cycle.
从石莼的单倍体配子体和二倍体孢子体的不同生活史阶段中提取了可溶性蛋白质,约占细胞总蛋白的 15%。从配子体和二倍体孢子体中提取的蛋白质提取物的电泳带图谱相同,除了在配子体中存在一条移动缓慢的带,但在二倍体孢子体中不存在。这条带也不存在于单倍体的营养孢子体的提取物中,但在游动孢子的提取物中可以看到。研究发现,这条带中的蛋白质在减数分裂前的大部分准备期内合成。在配子体中,这条带在配子的提取物中没有出现,因此可以推断,在配子体的有丝分裂配子囊分裂之前,这种蛋白质就被分解了。到目前为止,组成这条带的蛋白质的行为就像是决定生活史中世代转换的因素。