Division of Chemical Technology, C.S.I.R.O., 3205, South Melbourne, Victoria, Australia.
Planta. 1976 Jan;130(3):339-40. doi: 10.1007/BF00387843.
When cambial tissues are removed from tree stems of Eucalyptus regnans F. Muell. and incubated in vitro with [(14)C]glucose, [(14)C]galactose and [(14)C]fructose in the presence of sufficient PVP (polyvinylpyrrolidone) radioactive sucrose, members of the raffinose family and related sugars are synthesized. These results suggest that PVP inactivates substances which, in in vitro experiments, inhibit the action of enzymes which are essential to the resynthesis of nutrient sugars (sucrose, raffinose and stachyose). In living trees, such enzyme inhibition is less likely to occur.
当从巨桉(Eucalyptus regnans F. Muell.)树干的形成层组织中取出,并在有足够聚乙烯吡咯烷酮(PVP)存在的情况下,用[(14)C]葡萄糖、[(14)C]半乳糖和[(14)C]果糖进行体外培养时,会合成菜豆球蛋白族和相关糖。这些结果表明,PVP 使某些物质失活,而这些物质在体外实验中会抑制对营养糖(蔗糖、棉子糖和水苏糖)重新合成至关重要的酶的作用。在活树中,这种酶抑制不太可能发生。