Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, U.S.A..
Photosynth Res. 1989 Nov;22(2):123-30. doi: 10.1007/BF00035442.
Membranes and PS II particles retaining high rates of O2-evolving activity have been isolated from the transformable cyanobacterium, Synechocystis sp. PCC6803. Membranes from cells grown under red light exhibit rates of O2-evolution ranging from 500-700 μmole O2/mg chl/h. PS II particles are prepared by a simple procedure involving DEAE column chromatography of detergent extracts obtained by simultaneous treatment of membranes with octylglucoside and dodecylmaltoside. The isolated PS II fraction is enriched in polypeptides immunologically cross-reactive with polypeptides present in core reaction center preparations of spinach, exhibits 77 K fluorescence emission maxima at 685 and 696 nm, but not emission and absorption due to phycobilines and is capable of rates of O2-evolution exceeding 1000 μmole O2/mg chl/h.
已从可转化蓝藻集胞藻 PCC6803 中分离出保持高 O2 释放活性的膜和 PS II 颗粒。在红光下生长的细胞的膜表现出 500-700 μmole O2/mg chl/h 的 O2 释放速率。PS II 颗粒通过简单的程序制备，涉及用辛基葡糖苷和十二烷基麦芽糖同时处理膜获得的去污剂提取物的 DEAE 柱色谱法。分离的 PS II 级分富含与菠菜核心反应中心制剂中存在的多肽免疫交叉反应的多肽，在 685 和 696 nm 处表现出 77 K 荧光发射最大值，但没有由于藻胆蛋白而引起的发射和吸收，并且能够超过 1000 μmole O2/mg chl/h 的 O2 释放速率。
