Kronstrand Robert, Brinkhagen Linda, Birath-Karlsson Carolina, Roman Markus, Josefsson Martin
Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, 58758, Linköping, Sweden,
Anal Bioanal Chem. 2014 Jun;406(15):3599-609. doi: 10.1007/s00216-013-7574-x. Epub 2014 Jan 15.
The objective of this study was to compare the performance of an immunoassay screening for synthetic cannabinoids with a newly developed confirmation method using liquid chromatography quadrupole time-of-flight mass spectrometry. The screening included metabolites from JWH-018, JWH-073, and AM-2201. The confirmation included metabolites from AM-2201, JWH-018, JWH-019, JWH-073, JWH-081, JWH-122, JWH-210, JWH-250, JWH-398, MAM-2201, RCS-4, and UR-144. The immunoassay was tested and found to have no cross-reactivity with UR-144 metabolites but considerable cross-reactivity with MAM-2201 and JWH-122 metabolites. Sensitivity and specificity for the immunoassay were evaluated with 87 authentic urine samples and found to be 87% and 82%, respectively. With a cutoff at 2 ng/ml, the confirmation showed 80 positive findings in 38 cases. The most common finding was JWH-122 5-OH-pentyl, followed by JWH-018 5-OH-pentyl. There were 9 findings of UR-144 metabolites and 3 of JWH-073 metabolites. In summary, the immunoassay performed well, presenting both high sensitivity and specificity for the synthetic cannabinoids present in the urine samples tested. The rapid exchange of one cannabinoid for another may pose problems for immunoassays as well as for confirmation methods. However, we consider time-of-flight mass spectrometry to be superior since new metabolites can be quickly included and identified.
本研究的目的是比较免疫分析法筛查合成大麻素的性能与使用液相色谱四极杆飞行时间质谱法新开发的确证方法。筛查包括JWH-018、JWH-073和AM-2201的代谢物。确证包括AM-2201、JWH-018、JWH-019、JWH-073、JWH-081、JWH-122、JWH-210、JWH-250、JWH-398、MAM-2201、RCS-4和UR-144的代谢物。对免疫分析法进行了测试，发现其与UR-144代谢物无交叉反应，但与MAM-2201和JWH-122代谢物有相当程度的交叉反应。用87份真实尿液样本评估了免疫分析法的灵敏度和特异性，结果分别为87%和82%。以2 ng/ml为临界值，确证在38例中显示出80个阳性结果。最常见的结果是JWH-122 5-OH-戊基，其次是JWH-018 5-OH-戊基。有9个UR-144代谢物的结果和3个JWH-073代谢物的结果。总之，免疫分析法表现良好，对所测试尿液样本中的合成大麻素具有高灵敏度和特异性。一种大麻素快速被另一种取代可能给免疫分析法以及确证方法带来问题。然而，我们认为飞行时间质谱法更具优势，因为新的代谢物能够被快速纳入并鉴定出来。
