Jahr G, Gottschalk W
Institut für Genetik der Universität Bonn, BRD.
Theor Appl Genet. 1973 Jan;43(6):281-90. doi: 10.1007/BF00277789.
The germination of the pollen grains and the growth rate of the pollen tubes of eight mutants of Pisum sativum were compared with the parent line by in vitro investigations. All of the mutants studied showed a retardation of pollen tube growth as compared to the parent line, resulting in competitive elimination of some of the gametes in plants heterozygous for the respective mutant genes. The deficit of recessive plants in the progenies of heterozygous mutant strains of peas can be attributed to this retardation. Marked differences between the various mutants with regard to the levels of various free amino acids in their pollen grains were found. Certain amino acids may be present in greatly reduced concentration or may be present in excess. In some genotypes pollen tube growth can be stimulated by adding the deficient amino acid. This is especially true for proline, valine and threonine. As far as the other amino acids are concerned, the mutants studied showed varying reactions. The germination rate of the pollen grains is reduced by proline and threonine; germination is completely inhibited by glutamine. The amino acids isoleucine, histidine and cysteine retard pollen tube growth in all the mutants investigated. This is also true for leucine which, however, has a stimulating effect in one of the chlorophyll mutants.
通过体外研究，将豌豆（Pisum sativum）八个突变体的花粉粒萌发和花粉管生长速率与亲本系进行了比较。与亲本系相比，所有研究的突变体均表现出花粉管生长迟缓，导致在各自突变基因杂合的植物中一些配子被竞争性淘汰。豌豆杂合突变菌株后代中隐性植株的缺乏可归因于这种生长迟缓。在其花粉粒中各种游离氨基酸水平方面，不同突变体之间存在显著差异。某些氨基酸的浓度可能大大降低或可能过量存在。在某些基因型中，添加缺乏的氨基酸可刺激花粉管生长。脯氨酸、缬氨酸和苏氨酸尤其如此。就其他氨基酸而言，所研究的突变体表现出不同的反应。脯氨酸和苏氨酸会降低花粉粒的萌发率；谷氨酰胺会完全抑制萌发。异亮氨酸、组氨酸和半胱氨酸在所有研究的突变体中均会延缓花粉管生长。亮氨酸也是如此，不过，亮氨酸在其中一个叶绿素突变体中具有刺激作用。
