定量测定苏云金芽孢杆菌 GC-91 喷雾干燥制剂中的 δ-内毒素含量。

Quantitative determination of δ-endotoxin contents in spray-dried preparations ofBacillus thuringiensis strain GC-91.

机构信息

Agricultural Division, Ciba-Geigy Ltd, CH 4002, Basel, Switzerland.

出版信息

World J Microbiol Biotechnol. 1992 Jan;8(1):24-9. doi: 10.1007/BF01200679.

DOI:10.1007/BF01200679

Abstract

This report describes a method for quantifying δ-endotoxin contents in spray-dried preparations ofBacillus thuringiensis strain GC-91. δ-Endotoxin is solubilized in the pro-toxin form and separated from other soluble compounds by ion exchange chromatography. The method does not discriminate between the different δ-endotoxins produced by strain GC-91. It is suitable for quality control, since δ-endotoxin concentrations found in different preparations correlate inversely with the LC 50 in bioassays on artificial diet against freshly hatched larvae ofSpodoptera littoralis. A typical batch of spray-dried material contains 1.22%±0.08% δ-endotoxin. The method is most accurate with preparations containing 0.5 to 2.0% toxin, for which triplicate estimations give standard errors close to±0.2%.

摘要

本报告描述了一种定量测定苏云金芽孢杆菌 GC-91 菌株喷雾干燥制剂中 δ-内毒素含量的方法。δ-内毒素以原毒素的形式溶解，并通过离子交换层析与其他可溶性化合物分离。该方法不能区分 GC-91 菌株产生的不同 δ-内毒素。它适用于质量控制，因为在针对新孵化的地中海实蝇幼虫的人工饲料上进行的生物测定中，不同制剂中发现的 δ-内毒素浓度与 LC50 呈负相关。典型的一批喷雾干燥材料含有 1.22%±0.08%的 δ-内毒素。对于含有 0.5 至 2.0%毒素的制剂，该方法最准确，重复测定三次得到的标准误差接近±0.2%。