Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmacéuticas, Universidad de Chile, Casilla 233, Santiago 1, Chile.
World J Microbiol Biotechnol. 1992 May;8(3):319-23. doi: 10.1007/BF01201889.
Azotobacter vinelandii was better than eitherDerxia gummosa orRhizobium trifolii for sorption of UO 2 (2+) . Its maximum binding capacity was 0.25 mmol UO 2 (2+) /g dry biomass with an affinity constant of 333 l/mmol at pH 4.1 according to the Langmuir model. In a semisynthetic medium,A. vinelandii showed the highest sorption capacity in the early stationary phase. The binding of UO 2 (2+) , Cu(2+), Ca(2+) and Zn(2+) was affected by the pH of the solution. With HCl as eluent, virtually all the sorbed UO 2 (2+) was released. The presence of Cu(2+), Cd(2+), Ca(2+), and Zn(2+) inhibited the UO 2 (2+) biosorption whereas Mg(2+) and K(+) had no effect.
威兰氏固氮菌比胶状德克斯氏菌或三叶草根瘤菌更能吸附 UO 2 (2+) 。根据朗缪尔模型，其最大结合容量为 0.25mmol UO 2 (2+) /g 干生物质，在 pH4.1 时亲和力常数为 333l/mmol。在半合成培养基中，威兰氏固氮菌在早期静止期表现出最高的吸附能力。UO 2 (2+) 、Cu(2+) 、Ca(2+) 和 Zn(2+) 的结合受溶液 pH 的影响。用 HCl 作为洗脱剂，几乎可以释放所有吸附的 UO 2 (2+) 。Cu(2+) 、Cd(2+) 、Ca(2+) 和 Zn(2+) 的存在抑制了 UO 2 (2+) 的生物吸附，而 Mg(2+) 和 K(+) 则没有影响。
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