Institute of Microbiology, University of Lódź, Banacha 12/16, 90-237, Lódź, Poland.
World J Microbiol Biotechnol. 1992 Sep;8(5):500-4. doi: 10.1007/BF01201948.
Protoplasts ofCunninghamella elegans, showing 11α-, and 11β-hydroxylating ability of Substance S, preserved high transformation activity when dispersed in glucose-enriched, organic osmotic stabilizers. A joint action of polyoxins and 2-deoxy-D-glucose was necessary to prevent regeneration of the cell wall in long-lasting experiments. Stabilized and active, dispersed protoplasts may be an alternative research model for studying the function of the cell wall and intracellular metabolic pool constituents in steroid hydroxylation.
金孢子囊菌原生质体具有转化甾体物质 S 的 11α-和 11β-羟化能力，在富含葡萄糖的有机渗透稳定剂中分散时保持着较高的转化活性。聚多醇和 2-脱氧-D-葡萄糖的联合作用对于防止细胞壁在长时间实验中的再生是必需的。稳定且具有活性的分散原生质体可能是研究甾体羟化过程中细胞壁和细胞内代谢池成分功能的替代研究模型。
