Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India.
World J Microbiol Biotechnol. 1992 Nov;8(6):627-31. doi: 10.1007/BF01238802.
An alkalophilicBacillus (NCL-87-6-10, NCIM 2128), with a high productivity for extracellular xylanase (EC 3.2.1.8) and free of cellulase, was isolated from soil containing coconut fibre detritus. When grown on a wheat bran/yeast extract medium in submerged culture for 48 h, it produced 100 to 120 IU of enzyme activity per ml. The crude enzyme consists of two fractions of apparent mol sizes of approx 10.4 and 29 kDa in the proportion of 90:10, as determined by native gel exclusion chromatography. Optimum activity of the xylanase was at 60°C and pH 8.0. A two-fold increase in enzyme activity was obtained when reducing agents, thioethanol and dithiothreitol, were included in the assay.
从含有椰子纤维碎屑的土壤中分离到一株产碱性胞外木聚糖酶（EC 3.2.1.8）的嗜碱性芽孢杆菌（NCL-87-6-10，NCIM 2128），该酶不含纤维素酶。在含麦麸/酵母提取物的液体培养基中培养 48 小时，每毫升酶活可达 100-120IU。粗酶经天然凝胶排阻层析，显示两个主要活性组分的表观分子量分别约为 10.4 和 29 kDa，比例为 90:10。木聚糖酶的最适反应温度和 pH 值分别为 60°C 和 8.0。当在酶活测定体系中加入还原剂巯基乙醇和二硫苏糖醇时，酶活可提高两倍。
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