Ademiluyi Adedayo O, Oboh Ganiyu, Agbebi Oluwaseun J, Oyeleye Sunday I
Functional Foods, Nutraceuticals and Phytomedicine Unit, Department of Biochemistry, Federal University of Technology , Akure , Nigeria.
Pharm Biol. 2014 Jul;52(7):829-34. doi: 10.3109/13880209.2013.870585. Epub 2014 Jan 16.
Sorghum straw (dried leaves and stem fiber) extracts and infusion are employed in the management of several ailments in folklore, and it is also a natural dye source used in food preparation.
This study sought to investigate the modulatory effect of dietary inclusion of Sorghum straw dye on cisplatin-induced nephrotoxicity and antioxidant status in rats.
Adult male rats were randomly divided into four groups of six animals each. Groups I (normal rats) and II (control rats) were fed with basal diet while Groups III and IV were fed with diets containing 0.5% and 1% sorghum straw dye, respectively. Nephrotoxicity was induced in Groups I-IV on the 20th day by the administration of a single dose of cisplatin solution (7 mg/kg body weight, i.p.) and the experiment was terminated 3 d after. Thereafter, the kidney and plasma of the rats were analyzed for kidney function (creatinine, urea, uric acid, and blood urea nitrogen) and antioxidant indices [superoxide dismutase (SOD), catalase, glutathione-S-transferase (GST), malondialdehyde (MDA), vitamin C, and reduced glutathione (GSH)].
The average feed intake of the rats in all the groups ranged from 9.0 to 9.5 (g/rat/day). Furthermore, the result indicated that administration of cisplatin caused significant (p < 0.05) elevation in plasma creatinine (2.2 mg/dL), uric acid (39.3 mg/dL), urea (81.4 mg/dL), and blood urea nitrogen (38.0 mg/dL) as well as a concomitant decrease in kidney antioxidant indices in control rats as against the normal rats. However, diets supplemented with 0.5 and 1.0% sorghum straw dye significantly reversed the plasma creatinine and the kidney antioxidant indices to near normal levels.
The study suggests that dietary inclusion of sorghum straw dye as colorants could protect against oxidative stress and cisplatin-induced nephrotoxicity.
高粱秸秆（干叶和茎纤维）提取物及浸液在民间医学中用于治疗多种疾病，同时也是食品制备中使用的天然染料来源。
本研究旨在探讨日粮中添加高粱秸秆染料对顺铂诱导的大鼠肾毒性及抗氧化状态的调节作用。
成年雄性大鼠随机分为四组，每组六只。第一组（正常大鼠）和第二组（对照大鼠）饲喂基础日粮，而第三组和第四组分别饲喂含0.5%和1%高粱秸秆染料的日粮。在第20天，给第一至四组大鼠腹腔注射单剂量顺铂溶液（7 mg/kg体重）诱导肾毒性，实验在3天后结束。此后，分析大鼠的肾脏和血浆，检测肾功能（肌酐、尿素、尿酸和血尿素氮）及抗氧化指标[超氧化物歧化酶（SOD）、过氧化氢酶、谷胱甘肽-S-转移酶（GST）、丙二醛（MDA）、维生素C和还原型谷胱甘肽（GSH）]。
所有组大鼠的平均采食量在9.0至9.5（g/大鼠/天）之间。此外，结果表明，与正常大鼠相比，顺铂给药导致对照大鼠血浆肌酐（2.2 mg/dL）、尿酸（39.3 mg/dL）、尿素（81.4 mg/dL）和血尿素氮（38.0 mg/dL）显著升高（p < 0.05），同时肾脏抗氧化指标随之降低。然而，补充0.5%和1.0%高粱秸秆染料的日粮显著使血浆肌酐和肾脏抗氧化指标恢复至接近正常水平。
该研究表明，日粮中添加高粱秸秆染料作为着色剂可预防氧化应激和顺铂诱导的肾毒性。
