蜂王浆和绿茶提取物对顺铂诱导的小鼠肾毒性的保护作用：比较研究。
Protective effect of royal jelly and green tea extracts effect against cisplatin-induced nephrotoxicity in mice: a comparative study.
机构信息
Department of Pharmacology, Faculty of Health Sciences, Giresun University, Giresun, Turkey.
出版信息
J Med Food. 2009 Oct;12(5):1136-42. doi: 10.1089/jmf.2009.0036.
The aim of the present study was to investigate the protective role of royal jelly (RJ) and green tea (GT) extracts on cisplatin (cDDP)-induced nephrotoxicity in adult albino mice. Albino mice were randomly divided into six groups: Group I (control) received a single intraperitoneal injection of isotonic saline (0.02 mL/g), Group II received a single intraperitoneal injection of cDDP (7 mg/kg of body weight), Group III received RJ (100 mg/kg of body weight), Group IV received GT (100 mg/kg of body weight), Group V received RJ (100 mg/kg of body weight) + cDDP (7 mg/kg of body weight), and Group VI received GT (100 mg/kg of body weight) + cDDP (7 mg/kg of body weight). The concentrations of blood urea nitrogen (BUN) and creatinine were evaluated. In addition, kidney samples were taken for determination of tissue malondialdehyde (MDA) and reduced glutathione (GSH) levels. In addition, histopathological changes in kidneys were investigated. The results indicated that no significant differences in MDA, GSH, BUN, and creatinine levels were observed among the control group and groups treated with RJ alone and GT alone (P > .05). However, there was a significant increase in BUN and creatinine parameters after cDDP application in Groups II, V, and VI. The mice treated with only cDDP exhibited an increase in serum BUN and creatinine levels when compared to Groups V and VI (P < .05). Moreover, cDDP-induced oxidative damage caused a significant decrease in GSH levels and a significant increase in MDA levels in kidneys (P < .05). RJ and GT supplementation attenuated cDDP-induced nephrotoxicity, which was manifested by stopping the elevation in serum creatinine and BUN levels. Moreover, RJ and GT supplementation restored GSH content and MDA production levels in the kidney tissue following cDDP treatment (P < .05). These products were also effective in protecting against cDDP-induced tissue damage in mouse kidneys. In conclusion, 100 mg/kg of body weight doses of RJ and GT provided protection against cDDP-induced nephrotoxicity, and both products can act as protector agents against cDDP-induced kidney damages.
本研究旨在探讨蜂王浆（RJ）和绿茶（GT）提取物对顺铂（cDDP）诱导的成年白化小鼠肾毒性的保护作用。白化小鼠随机分为六组：第 I 组（对照组）接受单次腹腔注射等渗盐水（0.02 mL/g），第 II 组接受单次腹腔注射 cDDP（7 mg/kg 体重），第 III 组接受 RJ（100 mg/kg 体重），第 IV 组接受 GT（100 mg/kg 体重），第 V 组接受 RJ（100 mg/kg 体重）+cDDP（7 mg/kg 体重），第 VI 组接受 GT（100 mg/kg 体重）+cDDP（7 mg/kg 体重）。评估了血液尿素氮（BUN）和肌酐的浓度。此外，还采集了肾脏样本以测定组织丙二醛（MDA）和还原型谷胱甘肽（GSH）水平。此外，还研究了肾脏的组织病理学变化。结果表明，对照组和单独用 RJ 或 GT 处理的组之间 MDA、GSH、BUN 和肌酐水平无显著差异（P >.05）。然而，在仅用 cDDP 处理的组 II、V 和 VI 中，BUN 和肌酐参数显著增加。与 V 组和 VI 组相比，仅用 cDDP 处理的小鼠血清 BUN 和肌酐水平升高（P <.05）。此外，cDDP 诱导的氧化损伤导致肾脏中 GSH 水平显著降低，MDA 水平显著升高（P <.05）。RJ 和 GT 补充剂可减轻 cDDP 诱导的肾毒性，表现为停止血清肌酐和 BUN 水平升高。此外，RJ 和 GT 补充剂可恢复 cDDP 处理后肾脏组织中 GSH 含量和 MDA 产生水平（P <.05）。这些产品在保护小鼠肾脏免受 cDDP 诱导的组织损伤方面也很有效。总之，100 mg/kg 体重剂量的 RJ 和 GT 可提供对 cDDP 诱导的肾毒性的保护作用，并且这两种产品都可以作为对抗 cDDP 诱导的肾脏损伤的保护剂。
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