State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing, 210095, China.
J Integr Plant Biol. 2014 Jan;56(1):51-62. doi: 10.1111/jipb.12124.
Exploring the elite alleles and germplasm accessions related to fiber quality traits will accelerate the breeding of cotton for fiber quality improvement. In this study, 99 Gossypium hirsutum L. accessions with diverse origins were used to perform association analysis of fiber quality traits using 97 polymorphic microsatellite marker primer pairs. A total of 107 significant marker-trait associations were detected for three fiber quality traits under three different environments, with 70 detected in two or three environments and 37 detected in only one environment. Among the 70 significant marker-trait associations, 52.86% were reported previously, implying that these are stable loci for target traits. Furthermore, we detected a large number of elite alleles associated simultaneously with two or three traits. These elite alleles were mainly from accessions collected in China, introduced to China from the United States, or rare alleles with a frequency of less than 5%. No one cultivar contained more than half of the elite alleles, but 10 accessions were collected from China and the two introduced from the United States did contain more than half of these alleles. Therefore, there is great potential for mining elite alleles from germplasm accessions for use in fiber quality improvement in modern cotton breeding.
探索与纤维品质性状相关的优异等位基因和种质资源，将加速棉花纤维品质改良的育种进程。本研究利用 99 份具有不同起源的陆地棉（Gossypium hirsutum L.）种质资源，利用 97 对多态性微卫星标记引物对，进行纤维品质性状的关联分析。在三个不同环境下，共检测到三个纤维品质性状的 107 个显著标记-性状关联，其中 70 个在两个或三个环境中检测到，37 个仅在一个环境中检测到。在 70 个显著标记-性状关联中，有 52.86%是以前报道过的，表明这些是目标性状的稳定位点。此外，我们还检测到大量同时与两个或三个性状相关的优异等位基因。这些优异等位基因主要来自中国收集的、从美国引进到中国的或频率低于 5%的稀有等位基因。没有一个品种含有一半以上的优异等位基因，但有 10 个来自中国和两个从美国引进的品种含有一半以上的这些等位基因。因此，从种质资源中挖掘优异等位基因用于现代棉花育种中的纤维品质改良具有很大的潜力。
