A technique for simultaneous antikinetochore immunofluorescence staining and Q-banding in chromosomes from human lymphocytes.
作者信息
Cherry L M, Shah S A
出版信息
Stain Technol. 1987 Jul;62(4):221-5. doi: 10.3109/10520298709107998.
PMID:2442860
Abstract
Antikinetochore immunofluorescence staining has been used in several studies to determine whether a second kinetochore is present, active, or both, in multicentric chromosomes. All of these studies have used tissue culture cells, and contended with the problem of obtaining well spread, banded metaphase chromosomes without affecting the kinetochore staining. We have adapted hypotonic, centrifugation and chromosome staining techniques to obtain simultaneous Q-banding and bright kinetochore staining of chromosomes from human lymphocytes.
摘要
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