Kryukova Natalia A, Yurlova Natalia I, Rastyagenko Natalia M, Antonova Elena V, Glupov Viktor V
Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
J Parasitol. 2014 Jun;100(3):284-7. doi: 10.1645/13-214.1. Epub 2014 Jan 15.
The effects of trematode Plagiorchis mutationis parasitism on the cellular immune responses of the snail host Lymnaea stagnalis were investigated. The number of spreading blood cells (hemocytes) from infected snails was significantly less (69.5%) than in uninfected individuals (79.2%). The phagocytic activity of blood cells in infected snails was also significantly less (17.2%) compared to uninfected snails (27.8%). The hemocytes from the infected snails did not form a complete capsule around Sephadex beads in vitro. The protective reactions of the snail hosts were independent of the parasite load (daily cercariae production). In vitro, dead cercariae of P. mutationis were encapsulated by hemocytes from uninfected snails. The hemocytes of the infected snails formed a complete capsule around only 20% of dead cercariae in vitro, with remaining cercariae either unencapsulated (50% of cercariae) or incompletely encapsulated (30% of cercariae). The total number of hemocytes in the infected snails was twofold less than in uninfected individuals. The results of this study showed that the cellular response of snail host L. stagnalis to P. mutationis trematode infection is similar to the previously studied snail-trematode model systems.
研究了吸虫突变斜睾吸虫寄生对蜗牛宿主静水椎实螺细胞免疫反应的影响。受感染蜗牛的扩散血细胞（血细胞）数量（69.5%）显著低于未受感染个体（79.2%）。与未受感染的蜗牛（27.8%）相比，受感染蜗牛血细胞的吞噬活性也显著降低（17.2%）。受感染蜗牛的血细胞在体外不能围绕葡聚糖凝胶珠形成完整的包囊。蜗牛宿主的保护反应与寄生虫负荷（每日尾蚴产量）无关。在体外，未受感染蜗牛的血细胞能将突变斜睾吸虫的死尾蚴包囊起来。受感染蜗牛的血细胞在体外仅能将20%的死尾蚴包囊起来，其余尾蚴要么未被包囊（50%的尾蚴），要么被不完全包囊（30%的尾蚴）。受感染蜗牛的血细胞总数比未受感染个体少两倍。本研究结果表明，蜗牛宿主静水椎实螺对突变斜睾吸虫感染的细胞反应与先前研究的蜗牛 - 吸虫模型系统相似。
