白塞病患者血浆同型半胱氨酸水平与 HLA-B51 的关系：病例对照研究。

The relationship between plasma homocysteine level and HLA-B51 in patients with Behcet's disease: a case-control study.

机构信息

Rheumatology Department, Baqyiatallah University of Medical Sciences, Baghiatallah Hospital, Tehran, Iran; Behcet's Disease Unit, Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Int J Rheum Dis. 2014 May;17(4):466-70. doi: 10.1111/1756-185X.12271. Epub 2014 Jan 16.

DOI:10.1111/1756-185X.12271

PMID:24428874

Abstract

BACKGROUND

Various coagulation disorders have been reported to explain hypercoagulability state in Behcet's disease (BD). A possible negative association between human leukocyte antigen (HLA)-B51 and increased homocysteine level has been suggested in a previous report from Iranian patients with BD. The aim of this study was to find any possible relationship between plasma homocysteine levels and HLA-B51.

METHODS

In a case-control study, BD patients (fulfilling the new International Criteria for BD) and controls (who had similar clinical symptoms but BD was clinically excluded in them) were included. Mean plasma homocysteine levels measured by enzyme-linked immunosorbent assay in HLA-B51 positive and negative individuals both in patients and controls were compared by t-test, Mann-Whitney test and analysis of variance (F-test).

RESULTS

Ninety-six BD patients and 152 controls were recruited. There was no significant difference between HLA-B51 positive and negative individuals either in the mean plasma homocysteine levels (13.59 ± 9.03 vs. 12.95 ± 4.98 μmol/L, P = 0.514), or in the prevalence of hyperhomocysteinemia (17% vs. 21.4%, P = 0.504). This was true both for BD and control groups. In HLA-B51 positive and negative BD patients, mean plasma homocysteine levels were 14.29 ± 12.02 and 12.62 ± 4.79 μmol/L, respectively (P = 0.33), and the prevalence of hyperhomocysteinemia was 20.8% versus 19.5% (P = 0.55). In the control group, the mean plasma homocysteine levels in HLA-B51 positive and negative individuals were 12.85 ± 4.28 and 13.14 ± 5.10 μmol/L, respectively (P = 0.794), and the prevalence of hyperhomocysteinemia was 13% versus 22.1% (P = 0.23). The difference was non-significant regarding sex (P > 0.71) and disease activity (P > 0.31).

CONCLUSION

In contrast to our previous report, we found no relationship between plasma homocysteine levels and HLA-B51 in this study, either in BD or in the control group.

摘要

背景

已有报道称，各种凝血障碍可解释贝切特病（BD）中的高凝状态。此前伊朗 BD 患者的一项研究表明，人类白细胞抗原（HLA）-B51 与同型半胱氨酸水平升高之间可能存在负相关。本研究旨在探讨血浆同型半胱氨酸水平与 HLA-B51 之间是否存在任何可能的关系。

方法

采用病例对照研究，纳入符合新国际 BD 标准的 BD 患者（病例组）和具有相似临床症状但临床排除 BD 的患者（对照组）。采用酶联免疫吸附试验检测 HLA-B51 阳性和阴性个体的血浆同型半胱氨酸水平，采用 t 检验、Mann-Whitney 检验和方差分析（F 检验）比较两组间同型半胱氨酸水平的差异。

结果

共纳入 96 例 BD 患者和 152 例对照组。无论是在病例组还是对照组，HLA-B51 阳性和阴性个体的血浆同型半胱氨酸水平（分别为 13.59±9.03μmol/L 和 12.95±4.98μmol/L，P=0.514）或高同型半胱氨酸血症的发生率（分别为 17%和 21.4%，P=0.504）均无显著差异。在 HLA-B51 阳性和阴性的 BD 患者中，血浆同型半胱氨酸水平分别为 14.29±12.02μmol/L 和 12.62±4.79μmol/L（P=0.33），高同型半胱氨酸血症的发生率分别为 20.8%和 19.5%（P=0.55）。在对照组中，HLA-B51 阳性和阴性个体的血浆同型半胱氨酸水平分别为 12.85±4.28μmol/L 和 13.14±5.10μmol/L（P=0.794），高同型半胱氨酸血症的发生率分别为 13%和 22.1%（P=0.23）。性别（P＞0.71）和疾病活动度（P＞0.31）差异均无统计学意义。