Strand M, Dalton J P, Tom T D
Acta Trop Suppl. 1987 Jun;12:75-82.
In this report we have shown that mice vaccinated twice with radiation-attenuated cercariae elicit a much enhanced or unique response against six adult worm glycoproteins with molecular sizes of 200, 160, 140, 94, 58-56, and 43 kDa. In the case of the schistosomulum, vaccinated mice showed an enhanced or unique response to antigens of 200, 58, 46, 43, 25, and several glycoproteins in the range 65 to 50 kDa. That some or all of these antigens may be important for immunoprophylaxis against schistosomiasis is supported by the observations that 1. polyclonal antiserum (anti-IrV) prepared against these antigens also reacts with the major schistosomular surface antigens, and 2. this antiserum reacts with epitopes exposed on the surface of both newly transformed schistosomula and lung-stage schistosomula. In this study we also observed that the majority of the surface-iodinated antigens recognized by the anti-IrV serum were also recognized by sera from both vaccinated and patently infected mice. Simpson et al. (1985) have also shown that sera from vaccinated and infected mice recognized the same schistosomular surface antigens. It is possible, however, that the immune response of vaccinated mice is directed against different carbohydrate or peptide epitopes on these molecules, and that recognition of such epitopes is important for immune protection. Towards this goal we have cloned several schistosoma proteins reactive with the anti-IrV serum to identify peptide epitopes relevant for immunoprotection.
在本报告中，我们已表明，用辐射减毒尾蚴免疫两次的小鼠对六种分子大小分别为200、160、140、94、58 - 56和43 kDa的成虫糖蛋白引发了更强或独特的反应。就童虫而言，免疫小鼠对200、58、46、43、25 kDa的抗原以及65至50 kDa范围内的几种糖蛋白表现出增强或独特的反应。以下观察结果支持了这些抗原中的部分或全部可能对血吸虫病免疫预防很重要的观点：1. 针对这些抗原制备的多克隆抗血清（抗-IrV）也与主要的童虫表面抗原发生反应；2. 该抗血清与新转化的童虫和肺期童虫表面暴露的表位发生反应。在本研究中，我们还观察到抗-IrV血清识别的大多数表面碘化抗原也被免疫小鼠和显性感染小鼠的血清所识别。辛普森等人（1985年）也已表明，免疫小鼠和感染小鼠的血清识别相同的童虫表面抗原。然而，免疫小鼠的免疫反应有可能针对这些分子上不同的碳水化合物或肽表位，并且识别此类表位对免疫保护很重要。为实现这一目标，我们已克隆了几种与抗-IrV血清反应的血吸虫蛋白，以鉴定与免疫保护相关的肽表位。
