McNeill D L, Burden H W
Am J Anat. 1987 Jul;179(3):269-76. doi: 10.1002/aja.1001790308.
Afferent perikarya in dorsal root ganglia (DRG) at the T13 and L1 segmental levels projecting to the rat ovary were identified by utilizing the fluorescent retrograde tracer true blue (TB). Subsequently, TB-labeled ovarian afferent perikarya in DRG were examined for vasoactive intestinal polypeptide (VIP), substance P (SP), cholecystokinin-8 (CCK-8), neuropeptide Y (NPY), and somatostatin (SOM) immunoreactivity and for the presence of fluoride-resistant acid phosphatase (FRAP) enzyme activity. Of the ovarian afferent perikarya at the T13 and L1 segmental levels, 20.5% displayed VIP immunoreactivity, 23.8% displayed SP immunoreactivity, and 43.1% were immunoreactive for CCK-8. No ovarian afferent perikarya contained SOM or NYP immunoreactivity or FRAP activity. It is suggested that different neuropeptides may participate in modulation of specific ovarian functions.
利用荧光逆行示踪剂真蓝(TB)鉴定了T13和L1节段水平的背根神经节(DRG)中投射至大鼠卵巢的传入神经元胞体。随后,对DRG中TB标记的卵巢传入神经元胞体进行了血管活性肠肽(VIP)、P物质(SP)、胆囊收缩素-8(CCK-8)、神经肽Y(NPY)和生长抑素(SOM)免疫反应性检查以及耐氟酸性磷酸酶(FRAP)酶活性检测。在T13和L1节段水平的卵巢传入神经元胞体中,20.5%显示VIP免疫反应性,23.8%显示SP免疫反应性,43.1%对CCK-8呈免疫反应性。没有卵巢传入神经元胞体含有SOM或NYP免疫反应性或FRAP活性。提示不同的神经肽可能参与特定卵巢功能的调节。