嗜热碱性芽孢杆菌包埋法生产内切葡聚糖酶。

Endoglucanase production by entrappedBacillus thermoalkalophilus.

机构信息

Microbiology Unit, School of Life Sciences, Jawaharial Nehru University, 110 067, New Delhi, India.

出版信息

World J Microbiol Biotechnol. 1990 Sep;6(3):267-72. doi: 10.1007/BF01201295.

DOI:10.1007/BF01201295

Abstract

Endoglucanase (carboxymethylcellulase) production byBacillus thermoalkalophilus was both inducible and repressible. The enzyme production by free and immobilized cells was studied. Of the four entrapment matrices that were tried, polyacrylamide was the best at retaining cells at 60°C. The rate of CMCase production by immobilized cells was low initially, but increased and siabilized by day 7. Change in the metabolic behaviour of the immobilized cells was envisaged due to low oxygen consumption.

摘要

嗜热碱性杆菌的内切葡聚糖酶（羧甲基纤维素酶）的生产既具有诱导性又具有可阻遏性。研究了游离细胞和固定化细胞的产酶情况。在所尝试的四种包埋基质中，聚丙烯酰胺在 60°C 下最能保留细胞。固定化细胞的 CMCase 生产速率最初较低，但在第 7 天增加并稳定。由于耗氧量低，预计固定化细胞的代谢行为会发生变化。