从土曲霉中纯化糖化酶。
Purification of glucoamylase fromAspergillus terreus.
机构信息
Department of Biochemistry, University of Dhaka, 1000, Dhaka, Bangladesh.
出版信息
World J Microbiol Biotechnol. 1990 Dec;6(4):431-3. doi: 10.1007/BF01202129.
PMID:24430147
Abstract
Glucoamylase from a rice bran culture ofAspergillus terreus was purified by chromatography on DEAE-cellulose and concanavatin A-Sepharose. A homogenous monomer resulted after SDS-PAGE electrophoresis. The enzyme was a glycoprotein, molecular weight, 86,000 with 7.5% (w/w) carbohydrate content.
摘要
米曲霉麸曲来源的糖化酶经 DEAE-纤维素和刀豆球蛋白 A-Sepharose 柱层析纯化后，经 SDS-PAGE 电泳得到均一的单体。该酶是一种糖蛋白，分子量为 86000，含糖量为 7.5%（w/w）。
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