母犬输卵管和子宫液中的超氧化物歧化酶活性及其对犬精子体外活力、运动能力和超活化的影响。

Superoxide dismutase activity in the oviductal and uterine fluid of the bitch and the effects of the enzyme on viability, motility and hyperactivation of canine sperm in vitro.

作者信息

Kobayashi Masanori, Wada Miho, Hori Tatsuya, Kawakami Eiichi

机构信息

Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.

出版信息

J Vet Med Sci. 2014 May;76(5):741-3. doi: 10.1292/jvms.13-0545. Epub 2014 Jan 16.

DOI:10.1292/jvms.13-0545

PMID:24430658

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4073345/

Abstract

Superoxide dismutase (SOD) activity in flushings from oviducts and uterine horns of 8 anestrous, 5 estrous and 7 diestrous bitches was measured. SOD activity in oviductal fluid in estrous bitches was significantly higher than that in anestrous and diestrous bitches (P<0.01). SOD activity in uterine fluid of diestrous bitches was, however, significantly higher than that in anestrous and estrous bitches (P<0.01). Additionally, sperm collected from normal dogs were incubated in MEM and in MEM containing SOD (SOD-MEM) for 24 hr. The percentages of sperm with viability, motility and hyperactivation in SOD-MEM were higher than those in MEM. SOD produced in oviduct and uterus may be able to maintain or improve sperm quality and fertility in the dog.

摘要

测量了8只处于乏情期、5只处于发情期和7只处于间情期母犬输卵管和子宫角冲洗液中的超氧化物歧化酶（SOD）活性。发情期母犬输卵管液中的SOD活性显著高于乏情期和间情期母犬（P<0.01）。然而，间情期母犬子宫液中的SOD活性显著高于乏情期和发情期母犬（P<0.01）。此外，将从正常犬采集的精子在MEM和含有SOD的MEM（SOD-MEM）中孵育24小时。SOD-MEM中具有活力、运动能力和超活化能力的精子百分比高于MEM中的。输卵管和子宫中产生的SOD可能能够维持或改善犬的精子质量和生育能力。