Kobayashi Masanori, Wada Miho, Hori Tatsuya, Kawakami Eiichi
Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
J Vet Med Sci. 2014 May;76(5):741-3. doi: 10.1292/jvms.13-0545. Epub 2014 Jan 16.
Superoxide dismutase (SOD) activity in flushings from oviducts and uterine horns of 8 anestrous, 5 estrous and 7 diestrous bitches was measured. SOD activity in oviductal fluid in estrous bitches was significantly higher than that in anestrous and diestrous bitches (P<0.01). SOD activity in uterine fluid of diestrous bitches was, however, significantly higher than that in anestrous and estrous bitches (P<0.01). Additionally, sperm collected from normal dogs were incubated in MEM and in MEM containing SOD (SOD-MEM) for 24 hr. The percentages of sperm with viability, motility and hyperactivation in SOD-MEM were higher than those in MEM. SOD produced in oviduct and uterus may be able to maintain or improve sperm quality and fertility in the dog.
测量了8只处于乏情期、5只处于发情期和7只处于间情期母犬输卵管和子宫角冲洗液中的超氧化物歧化酶（SOD）活性。发情期母犬输卵管液中的SOD活性显著高于乏情期和间情期母犬（P<0.01）。然而，间情期母犬子宫液中的SOD活性显著高于乏情期和发情期母犬（P<0.01）。此外，将从正常犬采集的精子在MEM和含有SOD的MEM（SOD-MEM）中孵育24小时。SOD-MEM中具有活力、运动能力和超活化能力的精子百分比高于MEM中的。输卵管和子宫中产生的SOD可能能够维持或改善犬的精子质量和生育能力。
