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[抗生长素对伞藻(Bory et Mont.)Mont.离体组织片段再生过程中分生中心形成的影响]

[The effect of the antiauxin p-Chlorophenoxyisobutyric acid on the formation of meristematic centres during regeneration of isolated tissue fragments of Riella helicophylla (Bory et Mont.) Mont].

作者信息

Grotha R

机构信息

Arbeitsgruppe Pflanzenphysiologie, Gesamthochschule Kassel, Postfach, D-3500, Kassel, Federal Republic of Germany.

出版信息

Planta. 1976 Jan;129(3):235-8. doi: 10.1007/BF00398263.

Abstract

In isolated tissue fragments of Riella cultured in continuous light, p-Chlorophenoxyisobutyric acid (PCIB) strongly accelerates the polar cell divisions without changing the time of onset of the first nuclear divisions and totally inhibits the formation of unicellular rhizoids. No lateral meristems develop in the cauloids as they do in the control; instead the cells have uniform minimal size. Only preincubating the whole plant in PCIB before the fragments are cut results in a depolarisation with a much higher cell division activity. Preincubation of the whole plant followed by further incubation of the tissue fragments in PCIB decreases the area in which cell embryonization takes place, but the number of cell divisions therein increases to a still larger extent.

摘要

在持续光照下培养的莱氏藻离体组织片段中,对氯苯氧异丁酸(PCIB)强烈加速极性细胞分裂,而不改变首次核分裂开始的时间,并完全抑制单细胞假根的形成。茎状体中不形成像对照那样的侧生分生组织;相反,细胞具有均匀的最小尺寸。只有在切割组织片段之前将整株植物在PCIB中预培养,才会导致去极化并具有更高的细胞分裂活性。整株植物预培养后,再将组织片段在PCIB中进一步培养,会减少细胞胚胎发生的区域,但其中的细胞分裂数量增加得更多。

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