Frenkel C, Haard N F
Department of Horticulture and Forestry and Department of Food Science, Rutgers University, New Brunswick, New Jersey 08903.
Plant Physiol. 1973 Oct;52(4):380-4. doi: 10.1104/pp.52.4.380.
A vacuum infiltration technique was used to apply an anti-auxin, alpha-(p-chlorophenoxy) isobutyric acid to mature green pears (Pyrus communis var. Bartlett). Application of alpha-(p-chlorophenoxy) isobutyric acid, at 0.02, 0.2, and 2.0 mm progressively accelerated the onset of chlorophyll degradation, softening, and CO(2) evolution. The action of alpha(p-chlorophenoxy) isobutyric acid is apparently independent of ethylene, since the auxin analogue depressed ethylene evolution and could overcome ethylene deficiency in fruit ripening under hypobaric conditions.The auxin analogue decreased the Michaelis constant of indoleacetic acid oxidase in vitro, suggesting that the antiauxin action of alpha-(p-chlorophenoxy) isobutyric acid is the acceleration in the breakdown of endogenous auxins in fruit and subsequently the initiation of ripening.
采用真空渗透技术将抗生长素α-(对氯苯氧基)异丁酸施用于成熟的青皮梨(巴梨品种)。分别以0.02、0.2和2.0毫米的浓度施用α-(对氯苯氧基)异丁酸,能逐渐加速叶绿素降解、果实软化和二氧化碳释放的开始。α-(对氯苯氧基)异丁酸的作用显然与乙烯无关,因为这种生长素类似物能抑制乙烯释放,并且能克服减压条件下果实成熟过程中的乙烯缺乏。这种生长素类似物在体外降低了吲哚乙酸氧化酶的米氏常数,这表明α-(对氯苯氧基)异丁酸的抗生长素作用是加速果实内源性生长素的分解,进而启动成熟过程。