Department of Biological Sciences, University of Lancaster, LA1 4YQ, Lancaster, UK.
Photosynth Res. 1988 Apr;16(1-2):155-76. doi: 10.1007/BF00039491.
Manipulation of the CO2 concentration of the atmosphere allows the selection of photorespiratory mutants from populations of seeds treated with powerful mutagens such as sodium azide. So far, barley lines deficient in activity of phosphoglycolate phosphatase, catalase, the glycine to serine conversion, glutamine synthetase, glutamate synthase, 2-oxoglutarate uptake and serine: glyoxylate aminotransferase have been isolated. In addition one line of pea lacking glutamate synthase activity and one barley line containing reduced levels of Rubisco are available. The characteristics of these mutations are described and compared with similar mutants isolated from populations of Arabidopsis. As yet, no mutant lacking glutamine synthetase activity has been isolated from Arabidopsis and possible reasons for this difference between barley and Arabidopsis are discussed. The value of these mutant plants in the elucidation of the mechanism of photorespiration and its relationships with CO2 fixation and amino acid metabolism are highlighted.
大气中二氧化碳浓度的调节可从经强诱变剂（如叠氮化钠）处理的种子群体中选择出光呼吸突变体。迄今为止，已分离出缺乏磷酸甘油酸磷酸酶、过氧化氢酶、甘氨酸向丝氨酸转化、谷氨酰胺合成酶、谷氨酸合酶、2-酮戊二酸摄取和丝氨酸：乙醛酸氨基转移酶活性的大麦品系。此外，还获得了一种缺乏谷氨酸合酶活性的豌豆品系和一种Rubisco 水平降低的大麦品系。描述了这些突变的特征，并与从拟南芥群体中分离出的类似突变体进行了比较。到目前为止，尚未从拟南芥中分离出缺乏谷氨酰胺合成酶活性的突变体，讨论了大麦和拟南芥之间存在这种差异的可能原因。强调了这些突变体植物在阐明光呼吸机制及其与 CO2 固定和氨基酸代谢的关系方面的价值。
