Xiu R J, Duan C G, Mu G F
Microcirvalation Research Center, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1987 Mar;9(2):95-8.
Cheek pouches of Syrian golden hamsters and transparent access chambers of BALB/C mice were implanted with SP2/0 and HeLa tumor cells separately. Observation was done by continual photo and video from D 1 through the D 10 after implantation. It was found by the development of angiogenesis and the density of microvessels that both kinds of tumor cells could induce angiogenesis. On D 2 after implantation, there appeared leakage and hemorrhage from the microvessels near the tumor cells. On D 3 and D 4, there was an increased density of new capillaries which formed a very fine, tortuous and basketlike vascular plexus of irregular diameter. Most of the new microvessels came from the venules on the edge of the implant mass and they grew toward the tumor cells to penetrate the tumor tissue on D 4 to D 5. On D 7, the density of new microvessels reached the maximum and they began to extend outside the tumor which was surrounded by dense new capillaries. Compound 36 has been proved an effective substance against some tumors in clinical applications in this country. Also proved by our experiments, a drug effective in inhibition of angiogenesis induced by SP2/0 tumor cells.
分别将SP2/0和HeLa肿瘤细胞植入叙利亚金黄地鼠的颊囊和BALB/C小鼠的透明观察室。在植入后第1天至第10天通过连续拍照和录像进行观察。通过血管生成的发展和微血管密度发现，两种肿瘤细胞均可诱导血管生成。植入后第2天，肿瘤细胞附近的微血管出现渗漏和出血。在第3天和第4天，新生毛细血管密度增加，形成了直径不规则的非常细小、蜿蜒且呈篮状的血管丛。大多数新生微血管来自植入肿块边缘的小静脉，它们在第4天至第5天向肿瘤细胞生长并穿透肿瘤组织。在第7天，新生微血管密度达到最大值，它们开始向肿瘤外部延伸，肿瘤被密集的新生毛细血管包围。化合物36在我国临床应用中已被证明是一种对某些肿瘤有效的物质。我们的实验也证明，该药物对抑制SP2/0肿瘤细胞诱导的血管生成有效。
