Morsiani E, Mazzoni M, Ricci D, Gorini P
Institute of Surgical Pathology, University of Ferrara School of Medicine, Italy.
Eur Surg Res. 1987;19(5):305-11. doi: 10.1159/000128714.
Two different techniques of reconstruction after total gastrectomy were performed in rats: Roux-en-Y (RY) and jejunal interposition (JI). Sham-operated rats were used as controls. Sixteen weeks after surgery the animals were sacrificed. The distribution of the enteroendocrine cells in the duodenal and proximal jejunal mucosa was examined using the Grimelius silver nitrate stain. The frequency of the Grimelius-positive cells was estimated as number of cells per square millimeter of gut surface and as number of cells per transverse section of intestine. Intestinal villi height was also measured in the same duodenojejunal tracts. After gastrectomy, the JI rats showed a better weight gain than RY rats (p less than 0.05). A significant difference in the distribution of intestinal argyrophil cells was seen. The bypassed duodenojejunal tracts in RY rats showed a marked reduction of the Grimelius-positive cells in comparison with JI rats and controls (p less than 0.01). A reduction in the number of argyrophil cells in the proximal duodenum was also noticed in the JI rats in comparison with controls (p less than 0.01). A decrease in the villi height resulted in all the gastrectomized rats (p less than 0.01), but a significant decrease was also noticed in RY rats in comparison with JI (p less than 0.01). Our experimental observations suggested that the exclusion of the duodenum from intestinal transit after gastrectomy caused atrophy of the gut mucosa and a marked reduction in the number of argyrophil enteroendocrine cells in the bypassed tracts.
Roux-en-Y（RY）和空肠间置（JI）。假手术大鼠用作对照。术后16周处死动物。使用Grimelius硝酸银染色检查十二指肠和空肠近端黏膜中肠内分泌细胞的分布。将Grimelius阳性细胞的频率估计为每平方毫米肠表面的细胞数和每段肠横切面的细胞数。还在相同的十二指肠空肠段测量肠绒毛高度。胃切除术后，JI大鼠的体重增加优于RY大鼠（p<0.05）。观察到肠嗜银细胞分布存在显著差异。与JI大鼠和对照组相比，RY大鼠的旷置十二指肠空肠段Grimelius阳性细胞明显减少（p<0.01）。与对照组相比，JI大鼠的十二指肠近端嗜银细胞数量也减少（p<0.01）。所有胃切除大鼠的绒毛高度均降低（p<0.01），但与JI大鼠相比，RY大鼠的绒毛高度也显著降低（p<0.01）。我们的实验观察结果表明，胃切除术后十二指肠不参与肠道转运会导致肠黏膜萎缩以及旷置段嗜银肠内分泌细胞数量显著减少。
