[下调HE4基因表达对卵巢癌细胞生物学行为的影响]
[Effect of down-regulation of HE4 gene expression on biologic behavior of ovarian cancer cells].
作者信息
Zhou Lei, Xiao Ran, Chen Ying, Zhang Jing, Lu Ren-quan, Guo Lin
机构信息
Department of Clinical Laboratory, Cancer Center, Fudan University; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Department of Clinical Laboratory, Cancer Center, Fudan University; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. E-mail:
出版信息
Zhonghua Bing Li Xue Za Zhi. 2013 Oct;42(10):687-90.
OBJECTIVE
To investigate the effects of HE4 gene knockdown on the proliferation, adhesion and invasion of the ovarian cancer cells SKOV3.
METHODS
The knockdown of HE4 gene was performed by RNAi technology. The recombinant plasmids (pSUPER-HE4 shDNAs) were constructed and transfected into human ovarian cancer cells SKOV3. HE4 expression was then identified by real-time PCR and Western blot analysis. The invasion and adhesion ability of transduced cells were determined. In addition, cell proliferation and growth were analyzed by colonies formation assay.
RESULTS
Knockdown of HE4 was achieved, and further confirmed by real-time PCR and Western blot. The proliferation of HE4-down-regulated cells was not affected, but the invasion ability of the transfected cells was reduced (P < 0.05) and the adhesion ability was also reduced to 27.3%.
CONCLUSION
HE4 expression is down-regulated effectively by the constructed HE4 shDNA, and thus knockdown of HE4 inhibits the adhesion and invasion of SKOV3 cells.
目的
研究HE4基因敲低对卵巢癌细胞SKOV3增殖、黏附和侵袭的影响。
方法
采用RNAi技术敲低HE4基因。构建重组质粒（pSUPER-HE4 shDNAs）并转染至人卵巢癌细胞SKOV3。随后通过实时PCR和蛋白质印迹分析鉴定HE4表达。测定转导细胞的侵袭和黏附能力。此外，通过集落形成试验分析细胞增殖和生长情况。
结果
成功实现HE4基因敲低，并经实时PCR和蛋白质印迹进一步证实。HE4下调的细胞增殖未受影响，但转染细胞的侵袭能力降低（P < 0.05），黏附能力也降至27.3%。
结论
构建的HE4 shDNA有效下调了HE4表达，因此敲低HE4可抑制SKOV3细胞的黏附和侵袭。
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