Department of Animal Sciences, University of Florida, Gainesville, Florida, USA.
Rood and Riddle Equine Hospital, Lexington, Kentucky, USA.
Theriogenology. 2014 Mar 15;81(5):752-7. doi: 10.1016/j.theriogenology.2013.12.012. Epub 2013 Dec 21.
Supplementation with L-arginine can increase uterine arterial blood flow and vascular perfusion of the preovulatory follicle in mares. Increased vascular perfusion of the preovulatory follicle has been correlated with successful pregnancy in mares. The objective of this study was to determine if supplemental L-arginine would increase ovarian arterial blood flow, vascular perfusion of the preovulatory follicle, and embryo recovery rates in mares. Mares were blocked by age and breed and assigned at random within block to L-arginine supplementation or control groups. Mares were fed L-arginine beginning 17 days before and through the duration of the study. Transrectal Doppler ultrasonography was used to measure ovarian arterial blood flow and vascular perfusion of the preovulatory follicle daily when it reached 35 mm and subsequent CL on Days 2, 4, and 6. Mares, on achieving a follicle of 35 mm or more were bred via artificial insemination and an embryo collection was attempted 7 days after ovulation. Treatment did not affect interovulatory interval (arginine-treated, 18.1 ± 2.6 days; control, 20.7 ± 2.3 days) or embryo recovery rate (arginine-treated, 54%; control, 48%). Mares treated with l-arginine had a larger follicle for the 10 days preceding ovulation than control mares (30.4 ± 1.2 and 26.3 ± 1.3 mm, respectively; P < 0.05) and vascular perfusion of the dominant follicle tended (P = 0.10) to be greater for the 4 days before ovulation. No differences were observed between groups in diameter or vascular perfusion of the CL. Resistance indices, normalized to ovulation, were not significantly different between groups during the follicular or luteal phase. Oral l-arginine supplementation increased the size and tended to increase perfusion of the follicle 1, but had no effect on luteal perfusion or embryo recovery rates in mares.
精氨酸补充可以增加母马子宫动脉血流量和排卵前卵泡的血管灌注。排卵前卵泡的血管灌注增加与母马的成功妊娠有关。本研究的目的是确定补充精氨酸是否会增加母马的卵巢动脉血流量、排卵前卵泡的血管灌注和胚胎回收率。母马按年龄和品种分组，并在组内随机分配补充精氨酸或对照组。母马从研究开始前 17 天开始补充精氨酸，并持续到研究结束。当卵泡达到 35 毫米并在第 2、4 和 6 天达到随后的 CL 时，每天使用经直肠多普勒超声测量卵巢动脉血流量和排卵前卵泡的血管灌注。当母马的卵泡达到 35 毫米或更大时，通过人工授精进行配种，并在排卵后 7 天尝试进行胚胎采集。处理不影响排卵间隔（精氨酸处理，18.1 ± 2.6 天；对照，20.7 ± 2.3 天）或胚胎回收率（精氨酸处理，54%；对照，48%）。与对照组相比，接受 l-精氨酸处理的母马在排卵前 10 天的卵泡更大（分别为 30.4 ± 1.2 和 26.3 ± 1.3 毫米；P < 0.05），并且排卵前 4 天的优势卵泡的血管灌注也更大（P = 0.10）。在 CL 的直径或血管灌注方面，两组之间没有观察到差异。在卵泡期或黄体期，排卵后归一化的阻力指数在两组之间没有显著差异。口服 l-精氨酸补充增加了卵泡的大小，并倾向于增加卵泡 1 的灌注，但对黄体灌注或母马的胚胎回收率没有影响。
