State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-sen University, Guangzhou 510275, PR China.
State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-sen University, Guangzhou 510275, PR China.
Biochem Biophys Res Commun. 2014 Jan 31;444(1):86-91. doi: 10.1016/j.bbrc.2014.01.018. Epub 2014 Jan 14.
Transcriptome studies have revealed that many non-coding RNAs (ncRNAs) are located near the 3' sense terminus of protein-coding genes. However, the transcription and function of these RNAs remain elusive. Here, we identify a 3' sense termini-associated sRNA (TASR) downstream of rpl26 in Schizosaccharomyces pombe (S. pombe). Structure and function assays indicate that the TASR is an H/ACA box snoRNA required for 18S rRNA pseudouridylation at U121 and U305 sites and is therefore a cognate of snR49 from the budding yeast. Transcriptional studies show that pre-snR49 overlaps most of the coding sequence (CDS) of rpl26. Using scanning deletion analysis within promoter region, we show that the rpl26 promoter is required for the 3' TASR transcription. Interestingly, chromosomal synteny of rpl26-snR49 is found in the Schizosaccharomyces groups. Taken together, we have revealed a new transcriptional mechanism for 3' sense TASRs, which are transcribed by the same promoter as their upstream protein genes. These results further suggest that the origin and function of 3' sense ncRNAs are associated with upstream genes in higher eukaryotes.
转录组研究表明，许多非编码 RNA（ncRNA）位于蛋白质编码基因的 3' 正义末端附近。然而，这些 RNA 的转录和功能仍然难以捉摸。在这里，我们在裂殖酵母（Schizosaccharomyces pombe，S. pombe）中鉴定了 rpl26 下游的 3' 正义末端相关 sRNA（TASR）。结构和功能分析表明，TASR 是一种 H/ACA 盒 snoRNA，是 18S rRNA 在 U121 和 U305 位点假尿嘧啶化所必需的，因此是出芽酵母中 snR49 的同源物。转录研究表明，前 snR49 重叠 rpl26 编码序列（CDS）的大部分。通过启动子区域的扫描缺失分析，我们表明 rpl26 启动子是 3' TASR 转录所必需的。有趣的是，rpl26-snR49 在裂殖酵母群中存在染色体同线性。总之，我们揭示了一种新的 3' 正义 TASR 转录机制，它们由与上游蛋白质基因相同的启动子转录。这些结果进一步表明，3' 正义 ncRNA 的起源和功能与高等真核生物的上游基因有关。
