Kamat B R, Galli S J, Barger A C, Lainey L L, Silverman K J
Department of Pathology, Beth Israel Hospital, Boston, MA 02215.
Hum Pathol. 1987 Oct;18(10):1036-42. doi: 10.1016/s0046-8177(87)80220-4.
A new technique was developed for analyzing the neovascularization associated with coronary artery atherosclerosis: cinematography during silicone polymer injection of the coronary arteries of fixed and cleared human hearts, followed by histologic analysis in routine and 1-micron-thick, Epon-embedded sections. Twenty-two hearts obtained at autopsy were studied. On the basis of cinematographic findings, individual regions of the coronary arteries were classified as negative, positive, or abundantly positive for neovascularization. Positive and abundantly positive areas, which invariably occurred in segments exhibiting changes of atherosclerosis, contained numerous small vessels in the adventitia and outer media (4.7 +/- 1.5 and 9.8 +/- 1.3 [SE] vessel profiles/artery cross-section in positive and abundantly positive areas, versus 1.0 +/- 0.6 in negative regions). Abundantly positive areas, which occurred in coronary artery segments demonstrating the most extensive atherosclerotic change, contained numerous small vessels in the inner media or in the plaque itself. Some of these microvessels were in close proximity to mast cells, which represent potentially rich sources of mediators affecting vascular tone and permeability. Vessels were not observed in the inner media or in atherosclerotic plaque in areas designated either positive or negative by cinematography. These findings show how our approach can be used both to define the three-dimensional, in situ configuration of coronary artery neovascularization and to characterize the histology of this process in detail. They also confirm previous work indicating that areas of coronary arteries involved by atherosclerosis frequently exhibit extensive neovascularization.
对固定并清理后的人类心脏的冠状动脉进行硅聚合物注射时进行摄影，随后在常规的和1微米厚的环氧树脂包埋切片上进行组织学分析。研究了22例尸检获得的心脏。根据摄影结果，冠状动脉的各个区域被分类为新生血管形成阴性、阳性或大量阳性。阳性和大量阳性区域总是出现在表现出动脉粥样硬化变化的节段中，在外膜和中膜外层含有大量小血管（阳性和大量阳性区域分别为4.7±1.5和9.8±1.3[标准误]个血管轮廓/动脉横截面，而阴性区域为1.0±0.6）。大量阳性区域出现在显示最广泛动脉粥样硬化变化的冠状动脉节段中，在内膜或斑块本身中含有大量小血管。其中一些微血管紧邻肥大细胞，肥大细胞是影响血管张力和通透性的潜在丰富介质来源。在摄影分类为阳性或阴性的区域，在内膜或动脉粥样硬化斑块中未观察到血管。这些发现表明我们的方法如何既能用于定义冠状动脉新生血管形成的三维原位结构，又能详细描述这一过程的组织学特征。它们还证实了先前的研究工作，即动脉粥样硬化累及的冠状动脉区域经常表现出广泛的新生血管形成。
