Rizzoli R, Bonjour J P
Department of Medicine, University Hospital, Geneva, Switzerland.
J Cell Physiol. 1987 Sep;132(3):517-23. doi: 10.1002/jcp.1041320314.
The influence of the glucocorticoid dexamethasone on the cAMP response to parathyroid hormone (PTH) and various agonists was studied in epithelial monolayers of opossum kidney (OK) cells. The incubation with dexamethasone for 72 hours led to a dose-dependent higher cAMP response to PTH or forskolin in intact cells as well as in digitonin-permeabilized cells. This effect did not appear to result from changes in phosphodiesterase (PDE) activity nor from alterations in cAMP efflux from the cells. Moreover, dexamethasone increased the formation of domes by OK cell epithelium. Thus, dexamethasone seems to promote a more differentiated renal epithelial phenotype as suggested by enhanced hormonal response.
在负鼠肾（OK）细胞的上皮单层中研究了糖皮质激素地塞米松对环磷酸腺苷（cAMP）对甲状旁腺激素（PTH）及各种激动剂反应的影响。用地塞米松孵育72小时导致完整细胞以及洋地黄皂苷通透化细胞对PTH或福斯高林的cAMP反应呈剂量依赖性升高。这种效应似乎不是由磷酸二酯酶（PDE）活性的变化引起的，也不是由细胞内cAMP流出的改变引起的。此外，地塞米松增加了OK细胞上皮形成穹顶的能力。因此，如增强的激素反应所表明的那样，地塞米松似乎促进了更分化的肾上皮表型。
