Kim Bong-Suk, Halk Edward L, Merlo Donald J, Nelson Steven E, Loesch-Fries L Sue
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA.
Arch Virol. 2014 Jul;159(7):1787-91. doi: 10.1007/s00705-013-1945-7. Epub 2014 Jan 17.
The 32-kDa movement protein, P3, of alfalfa mosaic virus (AMV) is essential for cell-to-cell spread of the virus in plants. P3 shares many properties with other virus movement proteins (MPs); however, it is not known if P3 is posttranslationally modified by phosphorylation, which is important for the function of other MPs. When expressed in Nicotiana tabacum, P3 accumulated primarily in the cell walls of older leaves or in the cytosol of younger leaves. When expressed in Pischia pastoris, P3 accumulated primarily in a soluble form. Metabolic labeling indicated that a portion of P3 was phosphorylated in both tobacco and yeast, suggesting that phosphorylation regulates the function of this protein as it does for other virus MPs.
苜蓿花叶病毒（AMV）的32 kDa运动蛋白P3对于该病毒在植物中的细胞间传播至关重要。P3与其他病毒运动蛋白（MPs）具有许多共同特性；然而，尚不清楚P3是否通过磷酸化进行翻译后修饰，而磷酸化对其他MPs的功能很重要。当在烟草中表达时，P3主要积累在老叶的细胞壁或幼叶的细胞质中。当在巴斯德毕赤酵母中表达时，P3主要以可溶形式积累。代谢标记表明，P3的一部分在烟草和酵母中均被磷酸化，这表明磷酸化像对其他病毒MPs一样调节该蛋白的功能。
