Department of Botany, University of Wisconsin, 430 Lincoln Drive, 53706, Madison, Wisconsin, USA.
Planta. 1975 Jan;124(3):231-44. doi: 10.1007/BF00388685.
An ultrastructural study was made of the leaf tissues of four species of plants in three genera with Crassulacean acid metabolism ("CAM" plants): Kalanchoë daigremontiana Hamet et Perrier, K. verticillata Elliot, Sedum rubrotinctum clausen and Crassula tetragona L. Microbodies similar in appearance, with fibrillar or granular nucleoids but no crystalline deposits, were present in the mesophyll of all four species. The microbodies resembled in size and abundance those of C3 plants more closely than those of C4 plants, both under long-day and short-day conditions. The reaction for catalase activity employing 3,3'-diaminobenzidine produced a heavy deposit in the microbodies; the reaction was blocked by the catalase inhibitor, aminotriazole.Some of the plants of the two species of Kalanchoë studied contain in the epidermal and mesophyll cells of the leaves and plantlets an organelle-like structure consisting of a hollow cylinder, 90-160 nm in diameter and up to 2 μm or more in length, around which 18-20 or more minute tubules are wound in a steep helix. The tubules are only ca. 9 nm in diameter, hence are much smaller than conventional microtubules. The cylinder and surrounding tubules, herein tentatively assigned the term "microcylinder" for convenience, may represent a product of viral infection, or may be an organelle that appears at certain stages of growth or under particular environmental conditions. In any case it may prove to be of considerable importance for investigators of CAM plant physiology.
对具有景天酸代谢（“CAM”植物）的三个属的四种植物的叶片组织进行了超微结构研究：大戟属的大戟（Kalanchoë daigremontiana Hamet et Perrier）、虎尾兰（K. verticillata Elliot）、景天属的费氏景天（Sedum rubrotinctum clausen）和伽蓝菜属的伽蓝菜（Crassula tetragona L.）。在所有四个物种的叶肉中都存在外观相似的微体，具有纤维状或颗粒状核，但没有结晶沉积物。微体的大小和丰度与 C3 植物更相似，而与 C4 植物则不相似，无论是在长日和短日条件下。使用 3,3'-二氨基联苯胺进行的过氧化物酶活性反应在微体中产生了大量沉积物；该反应被过氧化物酶抑制剂氨基三唑阻断。研究的两种大戟属植物的一些植物在叶片和小植株的表皮和叶肉细胞中含有一种类似细胞器的结构，由一个空心圆柱体组成，直径为 90-160nm，长达 2μm 或更长，周围缠绕着 18-20 个或更多的微小小管，以陡峭的螺旋形缠绕。小管的直径仅约 9nm，因此比常规微管小得多。圆柱体和周围的小管，本文为方便起见暂称为“微管”，可能代表病毒感染的产物，也可能是在某些生长阶段或特定环境条件下出现的细胞器。无论如何，它可能对 CAM 植物生理学的研究人员具有重要意义。
