Department of Physiology and Environmental Studies, University of Nottingham, School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, U.K..
Planta. 1975 Jan;125(1):15-24. doi: 10.1007/BF00388869.
Phosphoenolpyruvate (PEP) carboxylase extracted from etiolated and greening sugar cane (a Saccharum hybrid) displayed different properties in terms of DEAE-cellulose elution profile and activation by glucose-6-phosphate. During the first 20 hours of greening, no increase in extractable PEP carboxylase activity was observed, but ionic and allosteric properties of the enzyme changed, becoming similar to those found for the enzyme from light-grown cane. Density labelling studies with deuterium oxide provided no evidence for either de novo synthesis or turnover of the enzyme during this period, or during the following 24 hours of greening. These results are discussed in relation to enzyme control mechanisms and the development of the C4 pathway in photosynthesis.
从黄化和转绿的甘蔗（甘蔗杂种）中提取的磷酸烯醇丙酮酸（PEP）羧化酶，在 DEAE-纤维素洗脱特性和被葡萄糖-6-磷酸激活方面表现出不同的特性。在转绿的最初 20 小时内，未观察到可提取的 PEP 羧化酶活性增加，但酶的离子和变构特性发生了变化，变得与来自光生长甘蔗的酶相似。氘水密度标记研究未提供在此期间或随后 24 小时的转绿过程中酶的从头合成或周转的证据。这些结果与酶的控制机制和光合作用中 C4 途径的发展有关。
