Department of Physiology and Environmental Studeis, University of Nottingham, School of Agriculture, Sutton, LE12 5RD, Bonington Loughborough, UK.
Planta. 1974 Mar;117(1):67-73. doi: 10.1007/BF00388679.
Phosphoenolpyruvate (PEP) carboxylase has been isolated from etiolated and light-grown sugar cane, and a comparison made of the physical and kinetic characteristics of the enzymes. Differences were found in the DEAE-cellulose elution profiles, in saturation kinetics with PEP as variable substrate and with Mg(2+) as variable cofactor, in sensitivities to NaCl as an inhibitor and glucose-6-phosphate as an activator. The etiolated tissue form consistently showed properties resembling more closely PEP carboxylase from a C3 plant than from a C4 plant, with K m PEP and K0.5 Mg values lower than the green tissue form, and markedly less sensitivity to NaCl and glucose-6-phosphate.
磷酸烯醇丙酮酸（PEP）羧化酶已从黄化和光生长的甘蔗中分离出来，并对酶的物理和动力学特性进行了比较。在 DEAE-纤维素洗脱图谱、以 PEP 为可变底物和以 Mg2+为可变辅因子的饱和动力学、对 NaCl 的敏感性作为抑制剂和葡萄糖-6-磷酸作为激活剂方面存在差异。黄化组织形式始终表现出与更接近 C3 植物的 PEP 羧化酶而不是 C4 植物的 PEP 羧化酶的特性，其 K m PEP 和 K0.5 Mg 值低于绿色组织形式，对 NaCl 和葡萄糖-6-磷酸的敏感性明显降低。
