Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University, Tsutsumidori, 980, Sendai, Japan.
Planta. 1975 Jan;125(2):197-200. doi: 10.1007/BF00388705.
When cell suspension of soybean was cultured in the presence of colchicine (1.0x10(-4) to 1.0x10(-3) M), the degree of cell separation was greatly increased. This promotion of cell separation was accompanied with swelling of cells and modification of cell form. The treated suspension consisted predominantly of single cells and a few small aggregates, and could be repeatedly subcultured in the presence of 1.0x10(-4) M colchicine, but could not at 1.0x10(-3) M.
当细胞悬浮液在秋水仙素（1.0x10(-4) 至 1.0x10(-3) M）存在的情况下培养时，细胞分离的程度大大增加。这种细胞分离的促进伴随着细胞肿胀和细胞形态的改变。处理后的悬浮液主要由单个细胞和少量小聚集体组成，并且可以在 1.0x10(-4) M 秋水仙素存在的情况下反复传代培养，但在 1.0x10(-3) M 时则不行。
