Tate and Lyle Limited, Group Research and Development, P.O. Box 68, RG6 2BX, Reading, UK.
Planta. 1975 Jan;122(1):45-52. doi: 10.1007/BF00385403.
Leaf tissue of the panicoid grass Pennisetum purpureum (Schum) and of the chloridoid grass Muhlenbergia montana (Hitchcock) were fractionated to produce preparations enriched in the contents of mesophyll and bundle sheath cells. Sucrose phosphate synthetase and sucrose synthetase were found predominantly in the mesophyll tissues of both species, as was uridine-diphosphate-glucose pyrophosphorylase in Pennisetum. In Muhlenbergia this enzyme was more plentiful in the bundle sheath cell fraction.Starch synthetase and adenosine-diphosphate-glucose pyrophosphorylase were plentiful in bundle sheath extracts of both species but phosphorylase was associated with extracts of mesophyll cells. In no case was there a clear-cut compartmentation of enzymes between leaf fractions.
将 panicoid 草属的 Pennisetum purpureum(Schum)和 chloridoid 草属的 Muhlenbergia montana(Hitchcock)的叶片组织进行分离,以制备富含叶肉和维管束鞘细胞内容物的制剂。发现蔗糖磷酸合成酶和蔗糖合成酶主要存在于这两个物种的叶肉组织中,而尿苷二磷酸葡萄糖焦磷酸化酶则存在于 Pennisetum 中。在 Muhlenbergia 中,这种酶在维管束鞘细胞部分更为丰富。淀粉合成酶和腺苷二磷酸葡萄糖焦磷酸化酶在两个物种的维管束鞘提取物中含量丰富,但磷酸化酶与叶肉细胞的提取物相关。在任何情况下,叶片部分之间的酶都没有明显的区室化。