Burduk Pawel K, Bodnar Magdalena, Sawicki Piotr, Szylberg Łukasz, Wiśniewska Ewa, Kaźmierczak Wojciech, Martyńska Maria, Marszałek Andrzej
Department of Otolaryngology and Clinical Oncology Chair and Clinic of Otolaryngology and Department of Pathophysiology of Hearing and Balance System Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, Bydgoszcz, Poland.
Head Neck. 2015 Mar;37(3):418-22. doi: 10.1002/hed.23618. Epub 2014 Apr 3.
The matrix metalloproteinases (MMPs) and their tissue inhibitor of metalloproteinases (TIMPs) can decompose extracellular matrix (ECM) components and brake down basement membranes and, thus, promote tumor local invasion and metastasis.
We studied 41 patients with squamous cell carcinoma (SCC) of the oropharynx who underwent surgical treatment. Material was diagnosed by routine procedure and examined by immunohistochemical analysis and dot blot technique. We searched for correlations between expressions of MMPs, such as MMP-2 and MMP-9 and their tissue inhibitors TIMP-1 and TIMP-2 and treatment outcome.
We found cytoplasmic expression of analyzed proteins, both in cancer cells and tumor stroma. The expression of analyzed antigens was higher in patients with lymph node metastases comparing patients without lymph node involvement.
Our data suggest that microenvironment changes are one of key factors in tumor progression. Divergent expression of MMPs and their inhibitors might be used as prognostic factor of oropharyngeal carcinoma progression.
基质金属蛋白酶（MMPs）及其金属蛋白酶组织抑制剂（TIMPs）可分解细胞外基质（ECM）成分并破坏基底膜，从而促进肿瘤局部侵袭和转移。
我们研究了41例接受手术治疗的口咽鳞状细胞癌（SCC）患者。材料经常规程序诊断，并通过免疫组织化学分析和斑点印迹技术进行检测。我们寻找MMPs（如MMP-2和MMP-9）及其组织抑制剂TIMP-1和TIMP-2的表达与治疗结果之间的相关性。
我们在癌细胞和肿瘤基质中均发现了所分析蛋白质的细胞质表达。与无淋巴结受累的患者相比，有淋巴结转移的患者所分析抗原的表达更高。
我们的数据表明，微环境变化是肿瘤进展的关键因素之一。MMPs及其抑制剂的不同表达可能用作口咽癌进展的预后因素。
