Laboratory of Virology.
Department of Obstetrics and Gynecology.
J Infect Dis. 2014 Jul 15;210(2):209-13. doi: 10.1093/infdis/jiu036. Epub 2014 Jan 16.
We developed and morphologically characterized a human genital mucosa explant model (endocervix and ectocervix/vagina) to mimic genital herpes infections caused by herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Subsequent analysis of HSV entry receptor expression throughout the menstrual cycle in genital tissues was performed, and the evolution of HSV-1/-2 mucosal spread over time was assessed. Nectin-1 and -2 were expressed in all tissues during the entire menstrual cycle. Herpesvirus entry mediator expression was limited mainly to some connective tissue cells. Both HSV-1 and HSV-2 exhibited a plaque-wise mucosal spread across the basement membrane and induced prominent epithelial syncytia.
我们开发并形态学表征了一种人类生殖器黏膜外植体模型（子宫颈内口和子宫颈外口/阴道），以模拟单纯疱疹病毒 1 型（HSV-1）和 2 型（HSV-2）引起的生殖器疱疹感染。随后在整个月经周期中分析了生殖器组织中 HSV 进入受体的表达，并评估了 HSV-1/-2 黏膜随时间的扩散演变。在整个月经周期中，所有组织中均表达了神经纤毛蛋白 1 和 2。疱疹病毒进入介质的表达主要局限于一些结缔组织细胞。HSV-1 和 HSV-2 均表现出跨基底膜的斑块状黏膜扩散，并诱导明显的上皮合胞体。
