大鼠慢肌和快肌中慢钠通道失活的比较。
Comparison between slow sodium channel inactivation in rat slow- and fast-twitch muscle.
作者信息
Ruff R L, Simoncini L, Stühmer W
机构信息
Department of Neurology, Cleveland Veterans Administration Hospital, OH 44106.
出版信息
J Physiol. 1987 Feb;383:339-48. doi: 10.1113/jphysiol.1987.sp016412.
Abstract
- Voltage-clamp Na+ currents (INa) were studied in rat soleus slow-twitch muscle fibres at about 18 degrees C using the loose-patch-clamp technique. The maximum inward current density was produced by depolarizations to about -19 mV. 2. Fast inactivation was studied utilizing 20 ms conditioning potentials. INa was reduced by 50% with conditioning potentials to about -70 mV. 3. Changes in the conditioning membrane potential produced slow changes in the peak INa due to a slow inactivation process. INa was reduced by 50% at about -86 mV due to slow inactivation. 4. The mean maximum inward INa when slow inactivation was fully removed was 6.83 mA cm-2. 5. Due to the slow inactivation process, slow-twitch fibres were less susceptible to reduction in INa than fast-twitch fibres.
摘要
- 使用膜片钳技术，在约18℃的大鼠比目鱼肌慢肌纤维中研究电压钳制钠电流（INa）。最大内向电流密度是由去极化至约 -19 mV产生的。2. 利用20 ms的预处理电位研究快速失活。当预处理电位至约 -70 mV时，INa降低50%。3. 由于缓慢失活过程，预处理膜电位的变化使峰值INa产生缓慢变化。由于缓慢失活，在约 -86 mV时INa降低50%。4. 当缓慢失活完全消除时，平均最大内向INa为6.83 mA cm-2。5. 由于缓慢失活过程，慢肌纤维比快肌纤维对INa降低的敏感性更低。
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